Coexpression of YY1 is required to elaborate the effector functions controlled by PLZF in NKT cells

Patrick W. Darcy, Kangxin Jin, Louis Osorio, Lisa K. Denzin, Derek B. Sant’Angelo

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The promyelocytic leukemia zinc-finger transcription factor (PLZF) is essential for nearly all of the unique, innate-like functions and characteristics of NKT cells. It is not known, however, if the activity of PLZF is regulated by other factors. In this article, we show that the function of PLZF is completely dependent on the transcription factor Yin Yang 1 (YY1). Mouse NKT cells expressing wild-type levels of PLZF, but deficient for YY1, had developmental defects, lost their characteristic “preformed” mRNA for cytokines, and failed to produce cytokine protein upon primary activation. Immunoprecipitation experiments showed that YY1 and PLZF were coassociated. Taken together, these biochemical and genetic data show that the broadly expressed transcription factor, YY1, is required for the cell-specific “master regulator” functions of PLZF.

Original languageEnglish (US)
Pages (from-to)627-638
Number of pages12
JournalJournal of Immunology
Volume203
Issue number3
DOIs
StatePublished - Aug 1 2019

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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