Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)