Comparison of antioxidant activities of isoflavones from kudzu root (Pueraria lobata Ohwi)

M. Jun, H. Y. Fu, J. Hong, X. Wan, C. S. Yang, C. T. Ho

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Isoflavones and their glycosides were isolated from kudzu root, and formononetin-7-O-glucoside (ononin) was identified from the plant for the first time. Among isolated compounds, the antioxidant potency of 5 major compounds was determined using (1) 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay, (2) thiocyanate assay in the linoleic acid model system, and (3) lipoxygenase inhibition assay. The study indicates that 5 compounds act as free-radical scavengers and inhibit both linoleic acid peroxidation and lipoxygenase activity. The 3 different assays for determining antioxidant activity revealed that activity in one test did not necessarily correlate with activity in another test. Arachidonic acid release in the intact HL-29 cancer cell, biochanin A, showed strong inhibition of about 70%, at a concentration of 50 μM.

Original languageEnglish (US)
Pages (from-to)2117-2122
Number of pages6
JournalJournal of Food Science
Issue number6
StatePublished - Aug 2003

All Science Journal Classification (ASJC) codes

  • Food Science


  • Antioxidant activity
  • Arachidonic acid
  • DPPH
  • Isoflavone glycosides
  • Isoflavones
  • Kudzu
  • Lipoxygenase inhibition
  • Pueraria lobata Ohwi
  • Puerariae radix


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