Competition between bacteriophage f2 RNA and bacteriophage T4 messenger RNA

Emanuel Goldman, Harvey F. Lodish

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In an Escherichia coli cell-free protein synthesis assay, mRNA isolated from cells late after infection by phage T4 out-competes bacteriophage f2 RNA. Addition of a saturating or subsaturating amount of T4 mRNA inhibits translation of f2 RNA, while even an excess of f2 RNA has no effect on translation of T4 mRNA. Peptide mapping of reaction products labeled with formyl-[35S]-methionyl-tRNA was used to quantitate f2 and T4 protein products synthesized in the same reaction. We suggest that messenger RNA competition might be one mechanism by which T4 superinfection of cells infected with phage f2 blocks translation of f2 RNA and possibly host mRNA.

Original languageEnglish (US)
Pages (from-to)663-672
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume64
Issue number2
DOIs
StatePublished - May 19 1975
Externally publishedYes

Fingerprint

RNA Phages
Bacteriophage T4
Bacteriophages
Protein Biosynthesis
RNA
Messenger RNA
CD4-Positive T-Lymphocytes
Superinfection
Peptide Mapping
Transfer RNA
Proteins
Escherichia coli
Reaction products
Infection
Assays
Peptides

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

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abstract = "In an Escherichia coli cell-free protein synthesis assay, mRNA isolated from cells late after infection by phage T4 out-competes bacteriophage f2 RNA. Addition of a saturating or subsaturating amount of T4 mRNA inhibits translation of f2 RNA, while even an excess of f2 RNA has no effect on translation of T4 mRNA. Peptide mapping of reaction products labeled with formyl-[35S]-methionyl-tRNA was used to quantitate f2 and T4 protein products synthesized in the same reaction. We suggest that messenger RNA competition might be one mechanism by which T4 superinfection of cells infected with phage f2 blocks translation of f2 RNA and possibly host mRNA.",
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Competition between bacteriophage f2 RNA and bacteriophage T4 messenger RNA. / Goldman, Emanuel; Lodish, Harvey F.

In: Biochemical and Biophysical Research Communications, Vol. 64, No. 2, 19.05.1975, p. 663-672.

Research output: Contribution to journalArticle

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AU - Lodish, Harvey F.

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