Complementary approaches to gauge the bioavailability and distribution of ingested berry polyphenolics

Mary Ann Lila, David M. Ribnicky, Leonel E. Rojo, Patricio Rojas-Silva, Andrew Oren, Robert Havenaar, Elsa M. Janle, Ilya Raskin, Gad G. Yousef, Mary H. Grace

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Two different strategies for investigating the likely fate, after ingestion, of natural, bioactive berry constituents (anthocyanins and other non-nutritive flavonoids) are compared. A model of the human gastrointestinal tract (TIM-1) that mimicked the biological environment from the point of swallowing and ingestion through the duodenum, jejunum, and ileum (but not the colon) was used to monitor the stability and bioaccessibility of anthocyanins from both maqui berry and wild blueberry. TIM-1 revealed that most anthocyanins were bioaccessible between the second and third hours after intake. Alternatively, biolabeled anthocyanins and other flavonoids generated in vitro from berry and grape cell cultures were administered to in vivo (rodent) models, allowing measurement and tracking of the absorption and transport of berry constituents and clearance through the urinary tract and colon. The advantages and limitations of the alternative strategies are considered.

Original languageAmerican English
Pages (from-to)5763-5771
Number of pages9
JournalJournal of agricultural and food chemistry
Issue number23
StatePublished - Jun 13 2012

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)


  • artificial gastrointestinal tract
  • bioaccessibility
  • bioavailability
  • radiolabeling


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