Complete nucleotide sequence and genome organization of tobacco mosaic virus isolated from Vicia faba

Xueping Zhou, Chaoyang Xue, Qing Chen, Yijun Qi, Debao Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Based on reported TMV-U1 sequence, primers were designed and fragments covering the entire genome of TMV broad bean strain (TMV-B) were obtained with RT-PCR. These fragments were cloned and sequenced and the 5′ and 3′ end sequences of genome were confirmed with RACE. The complete sequence of TMV-B comprises 6 395 nucleotides (nt) and four open reading frames, which correspond to 126 ku (1 116 amino acids), 183 ku (1 616 amino acids), 30 ku (268 amino acids) and 17.5 ku proteins (159 amino acids). The complete nucleotide sequence of TMV-B is 99.4% identical to that of TMV-U1. The two virus isolates share the same sequence of 5′, 3′ non-coding region and 17.5 K ORF, and 6, 1 and 3 amino acid changes are found in 126 K protein, 54 K protein and 30 K protein, respectively. The possible mechanism on the infection of TMV-B in Vicia faba is discussed.

Original languageEnglish (US)
Pages (from-to)207-208
Number of pages2
JournalScience in China, Series C: Life Sciences
Volume43
Issue number2
StatePublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Environmental Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • Complete nucleotide sequence
  • Tobacco mosaic virus
  • Vicia faba

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