Complete sequence of a blaKPC-Harboring cointegrate plasmid isolated from Escherichia coli

Kalyan D. Chavda; Liang Chen; Michael R. Jacobs; Albert D. Rojtman; Robert A. Bonomo; Barry N. Kreiswirth

doi:10.1128/AAC.00041-15

Complete sequence of a bla_KPC-Harboring cointegrate plasmid isolated from Escherichia coli

Kalyan D. Chavda
, Liang Chen
, Michael R. Jacobs
, Albert D. Rojtman
, Robert A. Bonomo
, Barry N. Kreiswirth

Center for Discovery and Innovation

Hackensack Meridian School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Horizontal transfer of bla_KPC-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a bla_KPC-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how bla_KPC can be spread from a nonconjugative plasmid through cointegration.

Original language	English
Pages (from-to)	2956-2959
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	5
DOIs	https://doi.org/10.1128/AAC.00041-15
State	Published - May 1 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

Access to Document

10.1128/AAC.00041-15

Cite this

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title = "Complete sequence of a blaKPC-Harboring cointegrate plasmid isolated from Escherichia coli",

abstract = "Horizontal transfer of blaKPC-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a blaKPC-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how blaKPC can be spread from a nonconjugative plasmid through cointegration.",

author = "Chavda, \{Kalyan D.\} and Liang Chen and Jacobs, \{Michael R.\} and Rojtman, \{Albert D.\} and Bonomo, \{Robert A.\} and Kreiswirth, \{Barry N.\}",

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T1 - Complete sequence of a blaKPC-Harboring cointegrate plasmid isolated from Escherichia coli

AU - Chavda, Kalyan D.

AU - Chen, Liang

AU - Jacobs, Michael R.

AU - Rojtman, Albert D.

AU - Bonomo, Robert A.

AU - Kreiswirth, Barry N.

PY - 2015/5/1

Y1 - 2015/5/1

N2 - Horizontal transfer of blaKPC-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a blaKPC-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how blaKPC can be spread from a nonconjugative plasmid through cointegration.

AB - Horizontal transfer of blaKPC-harboring plasmids contributes significantly to the inter- and intraspecies spread of Klebsiella pneumoniae carbapenemase (KPC). Here we report the complete nucleotide sequence of a blaKPC-harboring IncFIA plasmid, pBK32533, from Escherichia coli. pBK32533 is a cointegrate plasmid comprising of a 72-kb sequence identical to that of the nonconjugative pBK30661 plasmid plus an additional 170-kb element that harbors the genes for plasmid transfer. pBK32533 demonstrates how blaKPC can be spread from a nonconjugative plasmid through cointegration.

UR - https://www.scopus.com/pages/publications/84931282030

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DO - 10.1128/AAC.00041-15

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SN - 0066-4804

VL - 59

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EP - 2959

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

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