Computational design of peptides that target transmembrane helices

Hang Yin, Joanna S. Slusky, Bryan W. Berger, Robin S. Walters, Gaston Vilaire, Rustem I. Litvinov, James D. Lear, Gregory A. Caputo, Joel S. Bennett, William F. DeGrado

Research output: Contribution to journalArticlepeer-review

223 Scopus citations

Abstract

A variety of methods exist for the design or selection of antibodies and other proteins that recognize the water-soluble regions of proteins; however, companion methods for targeting transmembrane (TM) regions are not available. Here, we describe a method for the computational design of peptides that target TM helices in a sequence-specific manner. To illustrate the method, peptides were designed that specifically recognize the TM helices of two closely related integrins (αIIbβ3 and (α vβ3) in micelles, bacterial membranes, and mammalian cells. These data show that sequence-specific recognition of helices in TM proteins can be achieved through optimization of the geometric complementarity of the target-host complex.

Original languageEnglish (US)
Pages (from-to)1817-1822
Number of pages6
JournalScience
Volume315
Issue number5820
DOIs
StatePublished - Mar 30 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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