Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants

Lu Wang; Dongmeng Li; Kezhen Yang; Xiaoyu Guo; Chao Bian; Takeshi Nishimura; Jie Le; Miyo Terao Morita; Dominique C. Bergmann; Juan Dong

doi:10.1038/s41467-021-27748-w

Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants

Lu Wang, Dongmeng Li, Kezhen Yang, Xiaoyu Guo, Chao Bian, Takeshi Nishimura, Jie Le, Miyo Terao Morita, Dominique C. Bergmann, Juan Dong

Waksman Institute of Microbiology

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

Abstract

Cell polarity is a fundamental feature underlying cell morphogenesis and organismal development. In the Arabidopsis stomatal lineage, the polarity protein BASL controls stomatal asymmetric cell division. However, the cellular machinery by which this intrinsic polarity site is established remains unknown. Here, we identify the PRAF/RLD proteins as BASL physical partners and mutating four PRAF members leads to defects in BASL polarization. Members of PRAF proteins are polarized in stomatal lineage cells in a BASL-dependent manner. Developmental defects of the praf mutants phenocopy those of the gnom mutants. GNOM is an activator of the conserved Arf GTPases and plays important roles in membrane trafficking. We further find PRAF physically interacts with GNOM in vitro and in vivo. Thus, we propose that the positive feedback of BASL and PRAF at the plasma membrane and the connected function of PRAF and GNOM in endosomal trafficking establish intrinsic cell polarity in the Arabidopsis stomatal lineage.

Original language	American English
Article number	7
Journal	Nature communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-021-27748-w
State	Published - Dec 2022

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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title = "Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants",

abstract = "Cell polarity is a fundamental feature underlying cell morphogenesis and organismal development. In the Arabidopsis stomatal lineage, the polarity protein BASL controls stomatal asymmetric cell division. However, the cellular machinery by which this intrinsic polarity site is established remains unknown. Here, we identify the PRAF/RLD proteins as BASL physical partners and mutating four PRAF members leads to defects in BASL polarization. Members of PRAF proteins are polarized in stomatal lineage cells in a BASL-dependent manner. Developmental defects of the praf mutants phenocopy those of the gnom mutants. GNOM is an activator of the conserved Arf GTPases and plays important roles in membrane trafficking. We further find PRAF physically interacts with GNOM in vitro and in vivo. Thus, we propose that the positive feedback of BASL and PRAF at the plasma membrane and the connected function of PRAF and GNOM in endosomal trafficking establish intrinsic cell polarity in the Arabidopsis stomatal lineage.",

author = "Lu Wang and Dongmeng Li and Kezhen Yang and Xiaoyu Guo and Chao Bian and Takeshi Nishimura and Jie Le and Morita, {Miyo Terao} and Bergmann, {Dominique C.} and Juan Dong",

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doi = "10.1038/s41467-021-27748-w",

language = "American English",

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T1 - Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants

AU - Wang, Lu

AU - Li, Dongmeng

AU - Yang, Kezhen

AU - Guo, Xiaoyu

AU - Bian, Chao

AU - Nishimura, Takeshi

AU - Le, Jie

AU - Morita, Miyo Terao

AU - Bergmann, Dominique C.

AU - Dong, Juan

PY - 2022/12

Y1 - 2022/12

N2 - Cell polarity is a fundamental feature underlying cell morphogenesis and organismal development. In the Arabidopsis stomatal lineage, the polarity protein BASL controls stomatal asymmetric cell division. However, the cellular machinery by which this intrinsic polarity site is established remains unknown. Here, we identify the PRAF/RLD proteins as BASL physical partners and mutating four PRAF members leads to defects in BASL polarization. Members of PRAF proteins are polarized in stomatal lineage cells in a BASL-dependent manner. Developmental defects of the praf mutants phenocopy those of the gnom mutants. GNOM is an activator of the conserved Arf GTPases and plays important roles in membrane trafficking. We further find PRAF physically interacts with GNOM in vitro and in vivo. Thus, we propose that the positive feedback of BASL and PRAF at the plasma membrane and the connected function of PRAF and GNOM in endosomal trafficking establish intrinsic cell polarity in the Arabidopsis stomatal lineage.

AB - Cell polarity is a fundamental feature underlying cell morphogenesis and organismal development. In the Arabidopsis stomatal lineage, the polarity protein BASL controls stomatal asymmetric cell division. However, the cellular machinery by which this intrinsic polarity site is established remains unknown. Here, we identify the PRAF/RLD proteins as BASL physical partners and mutating four PRAF members leads to defects in BASL polarization. Members of PRAF proteins are polarized in stomatal lineage cells in a BASL-dependent manner. Developmental defects of the praf mutants phenocopy those of the gnom mutants. GNOM is an activator of the conserved Arf GTPases and plays important roles in membrane trafficking. We further find PRAF physically interacts with GNOM in vitro and in vivo. Thus, we propose that the positive feedback of BASL and PRAF at the plasma membrane and the connected function of PRAF and GNOM in endosomal trafficking establish intrinsic cell polarity in the Arabidopsis stomatal lineage.

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JO - Nature communications

JF - Nature communications

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M1 - 7

ER -

Connected function of PRAF/RLD and GNOM in membrane trafficking controls intrinsic cell polarity in plants

Abstract

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