Connexin 43-Mediated Astroglial Metabolic Networks Contribute to the Regulation of the Sleep-Wake Cycle

Jerome Clasadonte, Eliana Scemes, Zhongya Wang, Detlev Boison, Philip G. Haydon

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Astrocytes produce and supply metabolic substrates to neurons through gap junction-mediated astroglial networks. However, the role of astroglial metabolic networks in behavior is unclear. Here, we demonstrate that perturbation of astroglial networks impairs the sleep-wake cycle. Using a conditional Cre-Lox system in mice, we show that knockout of the gap junction subunit connexin 43 in astrocytes throughout the brain causes excessive sleepiness and fragmented wakefulness during the nocturnal active phase. This astrocyte-specific genetic manipulation silenced the wake-promoting orexin neurons located in the lateral hypothalamic area (LHA) by impairing glucose and lactate trafficking through astrocytic networks. This global wakefulness instability was mimicked with viral delivery of Cre recombinase to astrocytes in the LHA and rescued by in vivo injections of lactate. Our findings propose a novel regulatory mechanism critical for maintaining normal daily cycle of wakefulness and involving astrocyte-neuron metabolic interactions. Astrocytes supply energy metabolites to neurons, but how this influences behavior is unclear. Clasadonte et al. demonstrate that delivery of lactate from astrocytes to neurons is required for normal orexinergic neuronal activity and hence daily cycles of wakefulness.

Original languageEnglish (US)
Pages (from-to)1365-1380.e5
Issue number6
StatePublished - Sep 13 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)


  • EEG
  • astrocytic metabolic networks
  • gap junction
  • lactate
  • orexin neuron
  • sleep-wake cycle


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