Regulation der Sauerstoff-Aufnahme, der Mikrozirkulation und der Glucose-Abgabe durch zirkulierendes Noradrenalin in der perfundierten Rattenleber

Translated title of the contribution: Control of Oxygen Uptake, Microcirculation and Glucose Release by Circulating Noradrenaline in Perfused Rat Liver

Karlheinz Beckh, Regina Otto, Sungchul Ji, Kurt Jungermann

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The effect of noradrenaline on oxygen uptake, on periportal and perivenous oxygen tension at surface acini, on microcirculation and on glucose output were studied in isolated rat livers perfused at constant flow with Krebs-Henseleit-hydrogen carbonate buffer containing 5mM glucose and 2mM lactate. 1) Noradrenaline at 1μM concentration caused a decrease in oxygen uptake, while at 0.1 μM it led to an increase. Both high and low doses of noradrenaline decreased the tissue surface oxygen tension in periportal and ü after a transient rise ü in perivenous areas. 2) Noradrenaline at an overall constant flow caused an increase of portal pressure and an alteration of the intrahepatic distribution of the perfusate: at the surface of the liver and in cross sections infused trypan blue led to only a slightly heterogeneous staining after a low dose of noradrenaline but to a clearly heterogeneous staining after a high dose. 3) Both high and low doses of noradrenaline stimulated glucose release. 4) All effects could be inhibited by the α-blocking agent phentolamine. In conclusion, control of hepatic oxygen consumption by circulating noradrenaline is a complex result of opposing hemodynamic and metabolic components: the microcirculatory changes inhibit oxygen uptake; they dominate after high catecholamine doses. The metabolic effects include a stimulation of oxygen utilization; they prevail at low catecholamine levels. The noradrenergic control of glucose release is also very complex, involving direct, metabolic and indirect, hemodynamic components.

Translated title of the contributionControl of Oxygen Uptake, Microcirculation and Glucose Release by Circulating Noradrenaline in Perfused Rat Liver
Original languageUndefined/Unknown
Pages (from-to)671-678
Number of pages8
JournalBiological Chemistry Hoppe-Seyler
Volume366
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Keywords

  • Rat liver
  • glucose output
  • microcirculation
  • noradrenaline
  • oxygen uptake
  • surface oxygen tension

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