Crystallization of myosin subfragment 1

TY - JOUR

T1 - Crystallization of myosin subfragment 1

AU - Rayment, I.

AU - Winkelmann, D. A.

PY - 1984

Y1 - 1984

N2 - Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract X-rays to at least 4.5-Å resolution. The subfragment 1 crystallizes in space group P212121 where a = 107 Å, b = 117 Å, and c = 278 Å. The cell dimensions and intensity distribution on X-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.

AB - Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract X-rays to at least 4.5-Å resolution. The subfragment 1 crystallizes in space group P212121 where a = 107 Å, b = 117 Å, and c = 278 Å. The cell dimensions and intensity distribution on X-ray diffraction photographs are consistent with two molecules in the crystallographic asymmetric unit. Electrophoretic analysis shows that the myosin subfragment 1 present in the crystals contains a 95-kilodalton heavy chain fragment and both the essential and regulatory light chains.

UR - http://www.scopus.com/inward/record.url?scp=0021170006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021170006&partnerID=8YFLogxK

U2 - 10.1073/pnas.81.14.4378

DO - 10.1073/pnas.81.14.4378

M3 - Article

C2 - 6589600

SN - 0027-8424

VL - 81

SP - 4378

EP - 4380

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 14 I

ER -