CtBP-independent repression in the Drosophila embryo

Yutaka Nibu, Kate Senger, Michael Steven Levine

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

There are three mechanisms of transcriptional repression in eukaryotes. The first is quenching, whereby repressors and activators co-occupy closely linked sites and then the repressor inhibits adjacent activators. The second is direct repression, in which repressors block the function of the core transcription complex. The third is competition, in which repressors compete with activators for a common DNA-binding site. Previous studies have shown that the Drosophila melanogaster CtBP corepressor (dCtBP) is essential for the quenching activity of three short-range sequence-specific repressors in the early Drosophila embryo: Krüppel, Knirps, and Snail. Here we demonstrate that dCtBP is dispensable for target enhancers that contain overlapping activator and repressor binding sites. However, it is essential when Krüppel and Knirps repressor sites do not overlap activator sites but are instead located adjacent to either activators or the core promoter. These findings provide evidence that competition is distinct from quenching and direct repression. Quenching and direct repression depend on dCtBP, whereas competition does not.

Original languageEnglish (US)
Pages (from-to)3990-3999
Number of pages10
JournalMolecular and cellular biology
Volume23
Issue number11
DOIs
StatePublished - Jun 1 2003
Externally publishedYes

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Co-Repressor Proteins
Drosophila melanogaster
Drosophila
Embryonic Structures
Binding Sites
Eukaryota
DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Nibu, Yutaka ; Senger, Kate ; Levine, Michael Steven. / CtBP-independent repression in the Drosophila embryo. In: Molecular and cellular biology. 2003 ; Vol. 23, No. 11. pp. 3990-3999.
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CtBP-independent repression in the Drosophila embryo. / Nibu, Yutaka; Senger, Kate; Levine, Michael Steven.

In: Molecular and cellular biology, Vol. 23, No. 11, 01.06.2003, p. 3990-3999.

Research output: Contribution to journalArticle

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