Cyclin-dependent kinase 6 is the principal target of p27/Kip1 regulation of the G1-phase traverse in 1,25-dihydroxyvitamin D3-treated HL60 cells

Qing Mei Wang, Xun Luo, George P. Studzinski

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71 Scopus citations

Abstract

Control or cell proliferation remains of intense interest in cancer research. In the 1,25-dihydroxyvitamin D3 HL60 cell system, G1 arrest has been shown to be mediated by elevated levels of p27/Kip1 protein. We show here that the main target of the elevated p27/Kip1 in this system is cyclin- dependent kinase (Cdk) 6. The activity of Cdk2 is also down-regulated, and this is associated with altered and reduced levels of cyclin E in the kinase complex. Paradoxically, the kinase activity of Cdk4 is elevated, in spite of an almost complete G1 block. These data show that the functions of Cdk4 and Cdk6 are not redundant and that Cdk6 and Cdk2 activities are regulated by 1,25-dihydroxyvitamin D3.

Original languageAmerican English
Pages (from-to)2851-2855
Number of pages5
JournalCancer Research
Volume57
Issue number14
StatePublished - Jul 15 1997

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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