D-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine

Kunihiro Kishida, Gustavo Martinez, Tetsuo Iida, Takako Yamada, Ronaldo Ferraris, Yukiyasu Toyoda

Research output: Contribution to journalArticle

Abstract

D-Allulose has been reported to have beneficial health effects. However, the transport system(s) mediating intestinal D-allulose transport has not yet been clearly identified. The aim of this study was to investigate whether intestinal D-allulose transport is mediated by glucose transporter type 5 (GLUT5). When D-allulose alone was gavaged, plasma D-allulose levels were dramatically higher in rats previously fed fructose. This suggests enhanced intestinal D-allulose absorption paralleled increases in GLUT5 expression observed only in fructose-fed rats. When D-allulose was gavaged with D-fructose, previously observed increases in plasma D-allulose levels were dampened and delayed, indicating D-fructose inhibited transepithelial D-allulose transport into plasma. Tracer D-[ 14 C]-fructose uptake rate was reduced to 54.8% in 50 mM D-allulose and to 16.4% in 50 mM D-fructose, suggesting D-allulose competed with D-[ 14 C]-fructose and the affinity of D-allulose for GLUT5 was lower than that of D-fructose. GLUT5 clearly mediates, likely at lower affinity relative to D-fructose, intestinal D-allulose transport.

Original languageEnglish (US)
Pages (from-to)604-608
Number of pages5
JournalFood Chemistry
Volume277
DOIs
StatePublished - Mar 30 2019

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Glucose Transporter Type 5
psicose
glucose transporters
Small Intestine
small intestine
Fructose
fructose
Substrates
Dilatation and Curettage
Plasmas
Rats

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science

Cite this

Kishida, Kunihiro ; Martinez, Gustavo ; Iida, Tetsuo ; Yamada, Takako ; Ferraris, Ronaldo ; Toyoda, Yukiyasu. / D-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine. In: Food Chemistry. 2019 ; Vol. 277. pp. 604-608.
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abstract = "D-Allulose has been reported to have beneficial health effects. However, the transport system(s) mediating intestinal D-allulose transport has not yet been clearly identified. The aim of this study was to investigate whether intestinal D-allulose transport is mediated by glucose transporter type 5 (GLUT5). When D-allulose alone was gavaged, plasma D-allulose levels were dramatically higher in rats previously fed fructose. This suggests enhanced intestinal D-allulose absorption paralleled increases in GLUT5 expression observed only in fructose-fed rats. When D-allulose was gavaged with D-fructose, previously observed increases in plasma D-allulose levels were dampened and delayed, indicating D-fructose inhibited transepithelial D-allulose transport into plasma. Tracer D-[ 14 C]-fructose uptake rate was reduced to 54.8{\%} in 50 mM D-allulose and to 16.4{\%} in 50 mM D-fructose, suggesting D-allulose competed with D-[ 14 C]-fructose and the affinity of D-allulose for GLUT5 was lower than that of D-fructose. GLUT5 clearly mediates, likely at lower affinity relative to D-fructose, intestinal D-allulose transport.",
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D-Allulose is a substrate of glucose transporter type 5 (GLUT5) in the small intestine. / Kishida, Kunihiro; Martinez, Gustavo; Iida, Tetsuo; Yamada, Takako; Ferraris, Ronaldo; Toyoda, Yukiyasu.

In: Food Chemistry, Vol. 277, 30.03.2019, p. 604-608.

Research output: Contribution to journalArticle

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