Design and implementation of a novel superfusion system for ex vivo characterization of neural tissue by dielectric spectroscopy (DS)

K. F. Dobiszewski, M. R. Shaker, C. Prodan, A. A. Hill

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Dielectric spectroscopy is a widely utilized electrophysiological characterization method. The obtained dielectric spectra and derived properties have the potential of providing significant information regarding changes in the physiological state of a biological system. However, since many of the dielectric properties are obtained in vitro from excised tissue far removed from physiological conditions, the value of the information obtained may be diminished. In this paper, we introduce a superfusion system that is designed to produce ex vivo dielectric spectroscopy measurements by providing the living tissue with a continuous and ample supply of nutrients and oxygen while removing metabolites and other waste. This superfusion system provides the convenience of in vitro measurement while concurrently producing results that can be more closely correlated with actual physiological changes in the biological system.

Original languageEnglish (US)
Pages (from-to)195-205
Number of pages11
JournalPhysiological Measurement
Volume32
Issue number2
DOIs
StatePublished - Feb 2011

All Science Journal Classification (ASJC) codes

  • Physiology (medical)
  • Biophysics
  • Physiology
  • Biomedical Engineering

Keywords

  • Dielectric spectroscopy
  • Ex vivo
  • Neural tissue
  • Superfusion

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