Design and validation of a bifunctional ligand display system for receptor targeting

Limor Chen, Amado J. Zurita, Peter U. Ardelt, Ricardo J. Giordano, Wadih Arap, Renata Pasqualini

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Here we developed a bacteriophage display particle designed to serve as a bifunctional entity that can target tumors while delivering an agent. We engineered a chimera phage vector containing a pIII-displayed αv integrins-targeting moiety and a pVIII-displayed streptavidin binding adaptor moiety. By using the chimeric phage particle, targeting of αv integrins on cells in culture and tumor-related blood vessels was shown through different applications, including luminescent quantum dots localization, surface plasmon resonance-based binding detection, and an in vivo tumor model. The strategy validated here will accelerate the discovery and characterization of receptor-ligand binding events in high throughput, and cell-specific delivery of diagnostics or therapeutics to organs of choice without the need for chemical conjugation.

Original languageEnglish (US)
Pages (from-to)1081-1091
Number of pages11
JournalCell Chemical Biology
Volume11
Issue number8
DOIs
StatePublished - Aug 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Clinical Biochemistry
  • Pharmacology

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