Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing

Liang Zhao, Xiang Wu, Xiaofeng Wang, Chunying Duan, Hongyan Wang, Amol Punjabi, Yang Zhao, Yuanwei Zhang, Zuoshang Xu, Haifeng Gao, Gang Han

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We designed a unimolecular hyperstar polymer for efficient small interfering RNA (siRNA) delivery that can be processed under repeated lyophilization and reconstitution without the need of any excipient. The hyperstar polymer contains a biodegradable hyperbranched core and is bound to siRNA through its thousands of cationic arms that radiate from its core. The siRNA/hyperstar complexes showed siRNA transfection efficiency that was superior to that of Lipofectamine2000 in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1), whose mutation causes familial amyotrophic lateral sclerosis. More importantly, hyperstar polymers as unimolecular containers minimized the multipolymer cross-interaction during lyophilization, and this maintained the uniquely high transfection efficiency of the siRNA/hyperstar complexes after repeated freeze-drying and reconstitution without the conventional need for excipient.

Original languageEnglish (US)
Pages (from-to)700-704
Number of pages5
JournalACS Macro Letters
Volume6
Issue number7
DOIs
StatePublished - Jul 18 2017
Externally publishedYes

Fingerprint

Excipients
RNA
Small Interfering RNA
Polymers
Containers
Drying
Genes

All Science Journal Classification (ASJC) codes

  • Materials Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Organic Chemistry

Cite this

Zhao, Liang ; Wu, Xiang ; Wang, Xiaofeng ; Duan, Chunying ; Wang, Hongyan ; Punjabi, Amol ; Zhao, Yang ; Zhang, Yuanwei ; Xu, Zuoshang ; Gao, Haifeng ; Han, Gang. / Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing. In: ACS Macro Letters. 2017 ; Vol. 6, No. 7. pp. 700-704.
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abstract = "We designed a unimolecular hyperstar polymer for efficient small interfering RNA (siRNA) delivery that can be processed under repeated lyophilization and reconstitution without the need of any excipient. The hyperstar polymer contains a biodegradable hyperbranched core and is bound to siRNA through its thousands of cationic arms that radiate from its core. The siRNA/hyperstar complexes showed siRNA transfection efficiency that was superior to that of Lipofectamine2000 in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1), whose mutation causes familial amyotrophic lateral sclerosis. More importantly, hyperstar polymers as unimolecular containers minimized the multipolymer cross-interaction during lyophilization, and this maintained the uniquely high transfection efficiency of the siRNA/hyperstar complexes after repeated freeze-drying and reconstitution without the conventional need for excipient.",
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Zhao, L, Wu, X, Wang, X, Duan, C, Wang, H, Punjabi, A, Zhao, Y, Zhang, Y, Xu, Z, Gao, H & Han, G 2017, 'Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing' ACS Macro Letters, vol. 6, no. 7, pp. 700-704. https://doi.org/10.1021/acsmacrolett.7b00242

Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing. / Zhao, Liang; Wu, Xiang; Wang, Xiaofeng; Duan, Chunying; Wang, Hongyan; Punjabi, Amol; Zhao, Yang; Zhang, Yuanwei; Xu, Zuoshang; Gao, Haifeng; Han, Gang.

In: ACS Macro Letters, Vol. 6, No. 7, 18.07.2017, p. 700-704.

Research output: Contribution to journalArticle

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AU - Zhao, Liang

AU - Wu, Xiang

AU - Wang, Xiaofeng

AU - Duan, Chunying

AU - Wang, Hongyan

AU - Punjabi, Amol

AU - Zhao, Yang

AU - Zhang, Yuanwei

AU - Xu, Zuoshang

AU - Gao, Haifeng

AU - Han, Gang

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