Developmental changes of aggrecan, versican and neurocan in the retina and optic nerve

Susanna Popp, Julie S. Andersen, Richard U. Margolis

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have used a monoclonal antibody to neurocan and specific polyclonal antibodies to the non-homologous glycosaminoglycan attachment regions of aggrecan and mRNA splice variants of versican to compare the localization and developmental changes of these structurally related hyaluronan-binding chondroitin sulfate proteoglycans in the rat retina and optic nerve. Staining for aggrecan and versican was first seen at embryonic day 16 in the optic nerve and retina, whereas neurocan was not detected in the embryonic eye. At postnatal day 0 (P0), β-versican staining is largely confined to the inner plexiform layer whereas α-versican is also apparent in the neuroblastic layer. Both aggrecan and, much more weakly, neurocan immunoreactivity is present throughout the neonatal retina. At P9, aggrecan and versican immunoreactivity is most intense in the inner and outer plexiform and ganglion cell layers, accompanied by diffuse staining in the inner and outer nuclear layers. Aggrecan and α-versican are also present throughout the optic nerve and disk, whereas β-versican and neurocan are confined to the laminar beams of the optic nerve. Between P0 and P9 there is a marked increase in β-versican expression in the inner and outer nuclear layers and in the outer plexiform layer, whereas there is only weak staining of neurocan in the inner plexiform and ganglion cell layers of P9 retina. By 1 month postnatal the staining pattern of the fully differentiated retinal layers is essentially identical to that seen in the adult, where there is strong aggrecan and α-versican immunoreactivity in the retina and optic nerve, whereas β-versican has essentially disappeared from the adult retina and, similarly to neurocan, is present only in the laminar beams of the optic nerve. The marked decrease of β-versican in the retina is consistent with >90% decrease in its concentration in brain during postnatal development, suggesting that the developmental time-course for these proteoglycans in retina parallels that seen in other areas of the central nervous system.

Original languageEnglish (US)
Pages (from-to)351-356
Number of pages6
JournalExperimental Eye Research
Volume79
Issue number3
DOIs
StatePublished - Sep 1 2004

Fingerprint

Neurocan
Versicans
Aggrecans
Optic Nerve
Retina
Staining and Labeling
Ganglia
Chondroitin Sulfate Proteoglycans
Optic Disk
Proteoglycans
Hyaluronic Acid
Glycosaminoglycans

All Science Journal Classification (ASJC) codes

  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Ophthalmology

Keywords

  • extracellular matrix
  • hyaluronan
  • immunocytochemistry
  • lamina cribrosa
  • optic nerve
  • proteoglycans
  • retinal development

Cite this

Popp, Susanna ; Andersen, Julie S. ; Margolis, Richard U. / Developmental changes of aggrecan, versican and neurocan in the retina and optic nerve. In: Experimental Eye Research. 2004 ; Vol. 79, No. 3. pp. 351-356.
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Developmental changes of aggrecan, versican and neurocan in the retina and optic nerve. / Popp, Susanna; Andersen, Julie S.; Margolis, Richard U.

In: Experimental Eye Research, Vol. 79, No. 3, 01.09.2004, p. 351-356.

Research output: Contribution to journalArticle

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