Differential gene regulation of GHSR signaling pathway in the arcuate nucleus and NPY neurons by fasting, diet-induced obesity, and 17β-estradiol

Ali Yasrebi, Anna Hsieh, Kyle J. Mamounis, Elizabeth A. Krumm, Jennifer A. Yang, Jason Magby, Pu Hu, Troy Roepke

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ghrelin's receptor, growth hormone secretagogue receptor (GHSR), is highly expressed in the arcuate nucleus (ARC) and in neuropeptide Y (NPY) neurons. Fasting, diet-induced obesity (DIO), and 17β-estradiol (E2) influence ARC Ghsr expression. It is unknown if these effects occur in NPY neurons. Therefore, we examined the expression of Npy, Agrp, and GHSR signaling pathway genes after fasting, DIO, and E2 replacement in ARC and pools of NPY neurons. In males, fasting increased ARC Ghsr and NPY Foxo1 but decreased NPY Ucp2. In males, DIO decreased ARC and NPY Ghsr and Cpt1c. In fed females, E2 increased Agrp, Ghsr, Cpt1c, and Foxo1 in ARC. In NPY pools, E2 decreased Foxo1 in fed females but increased Foxo1 in fasted females. DIO in females suppressed Agrp and augmented Cpt1c in NPY neurons. In summary, genes involved in GHSR signaling are differentially regulated between the ARC and NPY neurons in a sex-dependent manner.

Original languageEnglish (US)
Pages (from-to)42-56
Number of pages15
JournalMolecular and Cellular Endocrinology
Volume422
DOIs
StatePublished - Feb 15 2016

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Ghrelin Receptor
Arcuate Nucleus of Hypothalamus
Neuropeptide Y
Nutrition
Gene expression
Neurons
Estradiol
Fasting
Obesity
Diet
Genes

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Molecular Biology
  • Biochemistry

Cite this

Yasrebi, Ali ; Hsieh, Anna ; Mamounis, Kyle J. ; Krumm, Elizabeth A. ; Yang, Jennifer A. ; Magby, Jason ; Hu, Pu ; Roepke, Troy. / Differential gene regulation of GHSR signaling pathway in the arcuate nucleus and NPY neurons by fasting, diet-induced obesity, and 17β-estradiol. In: Molecular and Cellular Endocrinology. 2016 ; Vol. 422. pp. 42-56.
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Differential gene regulation of GHSR signaling pathway in the arcuate nucleus and NPY neurons by fasting, diet-induced obesity, and 17β-estradiol. / Yasrebi, Ali; Hsieh, Anna; Mamounis, Kyle J.; Krumm, Elizabeth A.; Yang, Jennifer A.; Magby, Jason; Hu, Pu; Roepke, Troy.

In: Molecular and Cellular Endocrinology, Vol. 422, 15.02.2016, p. 42-56.

Research output: Contribution to journalArticle

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AU - Yasrebi, Ali

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AU - Yang, Jennifer A.

AU - Magby, Jason

AU - Hu, Pu

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