Differential regulation of p75 and trkB mRNA expression after depolarizing stimuli or BDNF treatment in basal forebrain neuron cultures

Robert C. Elliott, Ira B. Black, Cheryl Dreyfus

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Extensive evidence suggests that BDNF regulates neural function and architecture after depolarization. Expression of BDNF is increased after depolarization, and the ability of BDNF to modulate synaptic function is well documented. To further investigate BDNF signaling after activity, we analyzed the effects of depolarization or BDNF treatment on receptor mRNA expression in cultured basal forebrain neurons. Levels of mRNA coding for the cognate BDNF receptor, trkB, as well as the common neurotrophin receptor, p75, were quantitated simultaneously using a sensitive solution hybridization technique. Depolarization or BDNF treatment increased p75 mRNA expression 94% and 195%, respectively. In contrast, trkB message decreased 23% after depolarization but was unchanged by BDNF treatment. Together, these changes resulted in significant increases in the p75/trkB ratio after depolarization or BDNF treatment that could alter BDNF binding or signal transduction. J. Neurosci. Res.

Original languageEnglish (US)
Pages (from-to)83-88
Number of pages6
JournalJournal of Neuroscience Research
Volume66
Issue number1
DOIs
StatePublished - Oct 1 2001

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Brain-Derived Neurotrophic Factor
Neurons
Messenger RNA
trkB Receptor
Basal Forebrain
Nerve Growth Factor Receptor
Signal Transduction

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

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title = "Differential regulation of p75 and trkB mRNA expression after depolarizing stimuli or BDNF treatment in basal forebrain neuron cultures",
abstract = "Extensive evidence suggests that BDNF regulates neural function and architecture after depolarization. Expression of BDNF is increased after depolarization, and the ability of BDNF to modulate synaptic function is well documented. To further investigate BDNF signaling after activity, we analyzed the effects of depolarization or BDNF treatment on receptor mRNA expression in cultured basal forebrain neurons. Levels of mRNA coding for the cognate BDNF receptor, trkB, as well as the common neurotrophin receptor, p75, were quantitated simultaneously using a sensitive solution hybridization technique. Depolarization or BDNF treatment increased p75 mRNA expression 94{\%} and 195{\%}, respectively. In contrast, trkB message decreased 23{\%} after depolarization but was unchanged by BDNF treatment. Together, these changes resulted in significant increases in the p75/trkB ratio after depolarization or BDNF treatment that could alter BDNF binding or signal transduction. J. Neurosci. Res.",
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Differential regulation of p75 and trkB mRNA expression after depolarizing stimuli or BDNF treatment in basal forebrain neuron cultures. / Elliott, Robert C.; Black, Ira B.; Dreyfus, Cheryl.

In: Journal of Neuroscience Research, Vol. 66, No. 1, 01.10.2001, p. 83-88.

Research output: Contribution to journalArticle

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