DNA Looping Facilitates Targeting of a Chromatin Remodeling Enzyme

Adam N. Yadon, Badri Nath Singh, Michael Hampsey, Toshio Tsukiyama

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


ATP-dependent chromatin remodeling enzymes are highly abundant and play pivotal roles regulating DNA-dependent processes. The mechanisms by which they are targeted to specific loci have not been well understood on a genome-wide scale. Here, we present evidence that a major targeting mechanism for the Isw2 chromatin remodeling enzyme to specific genomic loci is through sequence-specific transcription factor (TF)-dependent recruitment. Unexpectedly, Isw2 is recruited in a TF-dependent fashion to a large number of loci without TF binding sites. Using the 3C assay, we show that Isw2 can be targeted by Ume6- and TFIIB-dependent DNA looping. These results identify DNA looping as a mechanism for the recruitment of a chromatin remodeling enzyme and define a function for DNA looping. We also present evidence suggesting that Ume6-dependent DNA looping is involved in chromatin remodeling and transcriptional repression, revealing a mechanism by which the three-dimensional folding of chromatin affects DNA-dependent processes.

Original languageEnglish (US)
Pages (from-to)93-103
Number of pages11
JournalMolecular Cell
Issue number1
StatePublished - Apr 11 2013

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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