Downstream oligonucleotides strongly enhance the affinity of GMP to RNA primer-template complexes

Chun Pong Tam, Albert C. Fahrenbach, Anders Björkbom, Noam Prywes, Enver Izgu, Jack W. Szostak

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Origins of life hypotheses often invoke a transitional phase of nonenzymatic template-directed RNA replication prior to the emergence of ribozyme-catalyzed copying of genetic information. Here, using NMR and ITC, we interrogate the binding affinity of guanosine 5'-monophosphate (GMP) for primer-template complexes when either another GMP, or a helper oligonucleotide, can bind downstream. Binding of GMP to a primer-template complex cannot be significantly enhanced by the possibility of downstream monomer binding, because the affinity of the downstream monomer is weaker than that of the first monomer. Strikingly, GMP binding affinity can be enhanced by ca. 2 orders of magnitude when a helper oligonucleotide is stably bound downstream of the monomer binding site. We compare these thermodynamic parameters to those previously reported for T7 RNA polymerase-mediated replication to help address questions of binding affinity in related nonenzymatic processes.

Original languageEnglish (US)
Pages (from-to)571-574
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number2
DOIs
StatePublished - Jan 18 2017

Fingerprint

Guanosine Monophosphate
Oligonucleotides
RNA
Monomers
Catalytic RNA
Copying
Binding sites
Thermodynamics
Binding Sites
Nuclear magnetic resonance
RNA primers

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry

Cite this

Tam, Chun Pong ; Fahrenbach, Albert C. ; Björkbom, Anders ; Prywes, Noam ; Izgu, Enver ; Szostak, Jack W. / Downstream oligonucleotides strongly enhance the affinity of GMP to RNA primer-template complexes. In: Journal of the American Chemical Society. 2017 ; Vol. 139, No. 2. pp. 571-574.
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Downstream oligonucleotides strongly enhance the affinity of GMP to RNA primer-template complexes. / Tam, Chun Pong; Fahrenbach, Albert C.; Björkbom, Anders; Prywes, Noam; Izgu, Enver; Szostak, Jack W.

In: Journal of the American Chemical Society, Vol. 139, No. 2, 18.01.2017, p. 571-574.

Research output: Contribution to journalArticle

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AU - Fahrenbach, Albert C.

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AU - Szostak, Jack W.

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