Early agr activation correlates with vancomycin treatment failure in multi-clonotype MRSA endovascular infections

Wessam Abdelhady, Liang Chen, Arnold S. Bayer, Kati Seidl, Michael R. Yeaman, Barry N. Kreiswirth, Yan Q. Xiong

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Objectives: Persistent MRSA infections are especially relevant to endovascular infections and correlate with suboptimal outcomes. However, the virulence signatures of Staphylococcus aureus that drive such persistence outcomes are not well defined. In the current study, we investigated correlations between accessory gene regulator (agr) activation and the outcome of vancomycin treatment in an experimental model of infective endocarditis (IE) due to MRSA strains with different agr and clonal complex (CC) types. Methods: Twelve isolates with the four most common MRSA CC and agr types (CC5-agr II, CC8-agr I, CC30-agr III and CC45-agr I) were evaluated for heterogeneous vancomycin-intermediate S. aureus (hVISA), agr function, agrA and RNAIII transcription, agr locus sequences, virulence and response to vancomycin in the IE model. Results: Early agr RNAIII activation (beginning at 2 h of growth) in parallel with strong d-haemolysin production correlated with persistent outcomes in the IE model following vancomycin therapy. Importantly, such treatment failures occurred across the range of CC/agr types studied. In addition, these MRSA strains: (i) were vancomycin susceptible in vitro; (ii) were not hVISA or vancomycin tolerant; and (iii) did not evolve hVISA phenotypes or perturbed d-haemolysin activity in vivo following vancomycin therapy. Moreover, agr locus sequence analyses revealed no common point mutations that correlated with either temporal RNAIII transcription or vancomycin treatment outcomes, encompassing different CC and agr types. Conclusions: These data suggest that temporal agr RNAIII activation and agr functional profiles may be useful biomarkers to predict the in vivo persistence of endovascular MRSA infections despite vancomycin therapy.

Original languageEnglish (US)
Pages (from-to)1443-1452
Number of pages10
JournalJournal of Antimicrobial Chemotherapy
Volume70
Issue number5
DOIs
StatePublished - Oct 20 2014

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)
  • Pharmacology

Keywords

  • Antibiotics
  • Bacteria
  • Resistance

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