Effect of estrogen on global myocardial ischemia-reperfusion injury in female rats

Peiyong Zhai, Thomas E. Eurell, Robert Cotthaus, Elizabeth H. Jeffery, Janice M. Bahr, David R. Gross

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

We investigated the effects of estrogen on global myocardial ischemia-reperfusion injury in rats that were ovariectomized (Ovx), sham-operated, or ovariectomized and then given 17β-estradiol (E2β) supplementation (Ovx+E2β). Hearts were excised, cannulated, perfused with and then immersed in chilled (4°C) cardioplegia solution for 30 min, and then retrogradely perfused with warm (37°C), oxygenated Krebs-Henseleit bicarbonate buffer for 120 min. The coronary flow rate, first derivative of left ventricular pressure, and nitrite production were all significantly lower in Ovx than in sham-operated or Ovx+E2β hearts. However, coronary flow rates or nitrate production were not consistently different throughout the entire reperfusion period. Ca2+ accumulated more in Ovx rat hearts than in sham-operated or Ovx+E2β hearts, and mitochondrial respiratory function was lower in Ovx hearts than in hearts from the other two groups. Marked interstitial edema and contraction bands were seen in hematoxylineosin-stained sections of Ovx rat hearts but not in hearts from either of the other groups. Hematoxylin-basic fuchsinpicric acid-stained sections revealed fewer viable myocytes in hearts from the Ovx group than from the sham or Ovx+E2β group. Transmission electron microscopy demonstrated more severely damaged mitochondria and ultrastructural damage to myocytes in Ovx rat hearts. Our results indicate that estrogen plays a cardioprotective role in global myocardial ischemia-reperfusion injury in female rats.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume279
Issue number6 48-6
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Myocardial Reperfusion Injury
Reperfusion Injury
Myocardial Ischemia
Estrogens
Muscle Cells
Induced Heart Arrest
Ventricular Pressure
Hematoxylin
Bicarbonates
Nitrites
Transmission Electron Microscopy
Nitrates
Reperfusion
Estradiol
Edema
Mitochondria

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Zhai, Peiyong ; Eurell, Thomas E. ; Cotthaus, Robert ; Jeffery, Elizabeth H. ; Bahr, Janice M. ; Gross, David R. / Effect of estrogen on global myocardial ischemia-reperfusion injury in female rats. In: American Journal of Physiology - Heart and Circulatory Physiology. 2000 ; Vol. 279, No. 6 48-6.
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Effect of estrogen on global myocardial ischemia-reperfusion injury in female rats. / Zhai, Peiyong; Eurell, Thomas E.; Cotthaus, Robert; Jeffery, Elizabeth H.; Bahr, Janice M.; Gross, David R.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 279, No. 6 48-6, 01.12.2000.

Research output: Contribution to journalArticle

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