Effect of Saturated Very Long-Chain Fatty Acids on the Organization of Lipid Membranes: A Study Combining 2H NMR Spectroscopy and Molecular Dynamics Simulations

Adrian Paz Ramos, Patrick Lagüe, Guillaume Lamoureux, Michel Lafleur

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Little is known about the interaction of very long-chain saturated fatty acids (VLCFAs) with biological membranes. However, this could play an important role on interleaflet interactions and signal transduction mechanisms in cells. The aim of this work is to determine how VLCFA structurally adapts in fluid phospholipid bilayers, since both species must exhibit a significant hydrophobic mismatch. The membrane organization has been described by means of 2H NMR and molecular dynamics simulations. Our results show that the protonation state affects the position and order of free fatty acids (FFAs) in phospholipid membranes. It was shown that the protonated FFA-C24 carboxyl group is located slightly under the POPC head group and therefore its acyl chain can interact with the lipids of the opposite leaflet. This interdigitation of the end of the acyl chain causes a second plateau observed in SC-D profiles, a very unusual feature in lipid systems.

Original languageEnglish (US)
Pages (from-to)6951-6960
Number of pages10
JournalJournal of Physical Chemistry B
Volume120
Issue number28
DOIs
StatePublished - Jul 21 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Physical and Theoretical Chemistry

Fingerprint

Dive into the research topics of 'Effect of Saturated Very Long-Chain Fatty Acids on the Organization of Lipid Membranes: A Study Combining <sup>2</sup>H NMR Spectroscopy and Molecular Dynamics Simulations'. Together they form a unique fingerprint.

Cite this