Efficient assessment of developmental, surgical and pathological lymphangiogenesis using a lymphatic reporter mouse and its embryonic stem cells

TY - JOUR

T1 - Efficient assessment of developmental, surgical and pathological lymphangiogenesis using a lymphatic reporter mouse and its embryonic stem cells

AU - Hong, Mingu

AU - Jung, Eunson

AU - Yang, Sara

AU - Jung, Wonhyuek

AU - Seong, Young Jin

AU - Park, Eunkyung

AU - Bramos, Athanasios

AU - Kim, Kyu Eui

AU - Lee, Sunju

AU - Daghlian, George

AU - Seo, Jung In

AU - Choi, Inho

AU - Choi, In Seon

AU - Koh, Chester J.

AU - Kobielak, Agnieszka

AU - Ying, Qi Long

AU - Johnson, Maxwell

AU - Gardner, Daniel

AU - Wong, Alex K.

AU - Choi, Dongwon

AU - Hong, Young Kwon

N1 - Publisher Copyright: © 2016 Hong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.

AB - Several lymphatic reporter mouse lines have recently been developed to significantly improve imaging of lymphatic vessels. Nonetheless, the usage of direct visualization of lymphatic vessels has not been fully explored and documented. Here, we characterized a new Prox1-tdTomato transgenic lymphatic reporter mouse line, and demonstrated how this animal tool enables the researchers to efficiently assess developmental, surgical and pathological lymphangiogenesis by direct visualization of lymphatic vessels. Moreover, we have derived embryonic stem cells from this reporter line, and successfully differentiated them into lymphatic vessels in vivo. In conclusion, these experimental tools and techniques will help advance lymphatic research.

UR - https://www.scopus.com/pages/publications/84975521290

UR - https://www.scopus.com/pages/publications/84975521290#tab=citedBy

U2 - 10.1371/journal.pone.0157126

DO - 10.1371/journal.pone.0157126

M3 - Article

C2 - 27280889

SN - 1932-6203

VL - 11

JO - PLoS One

JF - PLoS One

IS - 6

M1 - e0157126

ER -