Efficient prime editing in two-cell mouse embryos using PEmbryo

TY - JOUR

T1 - Efficient prime editing in two-cell mouse embryos using PEmbryo

AU - Kim-Yip, Rebecca P.

AU - McNulty, Ryan

AU - Joyce, Bradley

AU - Mollica, Antonio

AU - Chen, Peter J.

AU - Ravisankar, Purnima

AU - Law, Benjamin K.

AU - Liu, David R.

AU - Toettcher, Jared E.

AU - Ivakine, Evgueni A.

AU - Posfai, Eszter

AU - Adamson, Britt

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/12

Y1 - 2024/12

N2 - Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice.

AB - Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequency across 13 substitution edits at 8 sites), enabling same-generation phenotyping of founders. Whole-genome sequencing reveals that mismatch repair inhibition increases off-target indels at low-complexity regions in the genome without any obvious phenotype in mice.

UR - https://www.scopus.com/pages/publications/85184182221

UR - https://www.scopus.com/pages/publications/85184182221#tab=citedBy

U2 - 10.1038/s41587-023-02106-x

DO - 10.1038/s41587-023-02106-x

M3 - Article

C2 - 38321114

SN - 1087-0156

VL - 42

SP - 1822

EP - 1830

JO - Nature biotechnology

JF - Nature biotechnology

IS - 12

M1 - 5855

ER -