EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Jean M. Parry; Nathalie V. Velarde; Ariel J. Lefkovith; Matthew H. Zegarek; Julie S. Hang; Jonathan Ohm; Richard Klancer; Rika Maruyama; Marina K. Druzhinina; Barth D. Grant; Fabio Piano; Andrew Singson

doi:https://doi.org/10.1016/j.cub.2009.09.015

EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Jean M. Parry, Nathalie V. Velarde, Ariel J. Lefkovith, Matthew H. Zegarek, Julie S. Hang, Jonathan Ohm, Richard Klancer, Rika Maruyama, Marina K. Druzhinina, Barth D. Grant, Fabio Piano, Andrew Singson

Rutgers, The State University

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.

Original language	American English
Pages (from-to)	1752-1757
Number of pages	6
Journal	Current Biology
Volume	19
Issue number	20
DOIs	https://doi.org/10.1016/j.cub.2009.09.015
State	Published - Nov 3 2009

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

Keywords

CELLCYCLE
DEVBIO

Access to Document

https://doi.org/10.1016/j.cub.2009.09.015

Cite this

@article{c28c27f1621b417891a16086fc479d5b,

title = "EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans",

abstract = "The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.",

keywords = "CELLCYCLE, DEVBIO",

author = "Parry, {Jean M.} and Velarde, {Nathalie V.} and Lefkovith, {Ariel J.} and Zegarek, {Matthew H.} and Hang, {Julie S.} and Jonathan Ohm and Richard Klancer and Rika Maruyama and Druzhinina, {Marina K.} and Grant, {Barth D.} and Fabio Piano and Andrew Singson",

note = "Funding Information: We would like to thank G. Seydoux, D. Shakes, and C. Rongo for helpful comments and discussions as well as for vectors and worm strains. We also would like to thank K. Oegema and A. Audhya for the mCherry vector, E. Kipreos for the CYB-1:GFP strain, as well as members of the Singson lab and Rutgers University C. elegans community for intellectual and experimental support. Work in the Singson lab, the Grant lab, and the Piano lab is supported by grants from the NIH (R01 HD054681, R01 GM067237, and R01 HD046236, respectively). We acknowledge the Mitani Lab, the National Bioresource Project for the Nematode (Japan), and the North American Caenorhabditis elegans Knockout Consortium for alleles of egg-4 and egg-5. The Caenorhabditis Genetics Center provided several strains.",

year = "2009",

month = nov,

day = "3",

doi = "https://doi.org/10.1016/j.cub.2009.09.015",

language = "American English",

volume = "19",

pages = "1752--1757",

journal = "Current biology : CB",

issn = "0960-9822",

publisher = "Cell Press",

number = "20",

}

TY - JOUR

T1 - EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

AU - Parry, Jean M.

AU - Velarde, Nathalie V.

AU - Lefkovith, Ariel J.

AU - Zegarek, Matthew H.

AU - Hang, Julie S.

AU - Ohm, Jonathan

AU - Klancer, Richard

AU - Maruyama, Rika

AU - Druzhinina, Marina K.

AU - Grant, Barth D.

AU - Piano, Fabio

AU - Singson, Andrew

N1 - Funding Information: We would like to thank G. Seydoux, D. Shakes, and C. Rongo for helpful comments and discussions as well as for vectors and worm strains. We also would like to thank K. Oegema and A. Audhya for the mCherry vector, E. Kipreos for the CYB-1:GFP strain, as well as members of the Singson lab and Rutgers University C. elegans community for intellectual and experimental support. Work in the Singson lab, the Grant lab, and the Piano lab is supported by grants from the NIH (R01 HD054681, R01 GM067237, and R01 HD046236, respectively). We acknowledge the Mitani Lab, the National Bioresource Project for the Nematode (Japan), and the North American Caenorhabditis elegans Knockout Consortium for alleles of egg-4 and egg-5. The Caenorhabditis Genetics Center provided several strains.

PY - 2009/11/3

Y1 - 2009/11/3

N2 - The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.

AB - The molecular underpinnings of the oocyte-to-embryo transition are poorly understood. Here we show that two protein tyrosine phosphatase-like (PTPL) family proteins, EGG-4 and EGG-5, are required for key events of the oocyte-to-embryo transition in Caenorhabditis elegans. The predicted EGG-4 and EGG-5 amino acid sequences are 99% identical and their functions are redundant. In embryos lacking EGG-4 and EGG-5, we observe defects in meiosis, polar body formation, the block to polyspermy, F-actin dynamics, and eggshell deposition. During oogenesis, EGG-4 and EGG-5 assemble at the oocyte cortex with the previously identified regulators or effectors of the oocyte-to-embryo transition EGG-3, CHS-1, and MBK-2 [1, 2]. All of these molecules share a complex interdependence with regards to their dynamics and subcellular localization. Shortly after fertilization, EGG-4 and EGG-5 are required to properly coordinate a redistribution of CHS-1 and EGG-3 away from the cortex during meiotic anaphase I. Therefore, EGG-4 and EGG-5 are not only required for critical events of the oocyte-to-embryo transition but also link the dynamics of the regulatory machinery with the advancing cell cycle.

KW - CELLCYCLE

KW - DEVBIO

UR - http://www.scopus.com/inward/record.url?scp=70350324714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350324714&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.cub.2009.09.015

DO - https://doi.org/10.1016/j.cub.2009.09.015

M3 - Article

C2 - 19879147

SN - 0960-9822

VL - 19

SP - 1752

EP - 1757

JO - Current biology : CB

JF - Current biology : CB

IS - 20

ER -

EGG-4 and EGG-5 Link Events of the Oocyte-to-Embryo Transition with Meiotic Progression in C. elegans

Abstract

ASJC Scopus subject areas

Keywords

Access to Document

Other files and links

Fingerprint

Cite this