Emergence of a new satellite RNA from cucumber mosaic virus isolate Pl

Sandra Perez Alvarez, Chao Yang Xue, Xue Ping Zhou

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The cucumber mosaic virus (CMV) isolate PI caused very mild symptoms on many plant species. After serial passages by mechanical inoculation over five years, CMV Pl caused severe symptoms on several tobacco cultivars and tomato. A specific band of approximately 0.3 kb in length was amplified by RT-PCR with primers synthesized based on reported CMV satellite RNA (satRNA) sequences. Sequence analysis showed there were two satRNAs (Sat-Pl-1 and Sat-Pl-2). Sat-Pl-1 contained 335 nucleotides, and Sat-Pl-2 contained 394 nucleotides. These two satRNAs shared 64% overall nucleotide sequence homology, and differences between the two satRNAs included mutations as well as deletions. Sat-Pl-1 was identical to a satRNA (Z96099) reported in 1995 in CMV PI. Based on differences in the sequence and secondary structure between these two satRNAs, we conclude that Sat-Pl-2 represents the emergence of a new satellite (necrotic satellite) from attenuated satRNA populations. The possible effect of the emergence of this new satRNA is discussed.

Original languageEnglish (US)
Pages (from-to)336-339
Number of pages4
JournalJournal of Zhejiang University: Science
Volume4
Issue number3
StatePublished - May 2003
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)

Keywords

  • Cucumber mosaic virus
  • Satellite RNA
  • Sequence

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