Endonuclease Activity Associated with the DNA · Protein Complex of Minicircular DNA of Escherichia coli 15

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Abstract

The DNA · protein complex (form I*) of minicircular DNA of Escherichia coli has associated with it an endonucleolytic activity which converts the supercoiled form I into an open circular form II by introducing one single‐stranded break per molecule. This nick can occur with similar frequency in either strand. Prolonged incubation results in the formation of linear duplex molecules (form III). The endonuclease requires a divalent cation (Mg2+ or Mn2+) and is inhibited by high salt concentration and transfer RNA. It might provide a swivel mechanism during the replication of minicircular DNA.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalEuropean Journal of Biochemistry
Volume36
Issue number1
DOIs
StatePublished - Jul 1973

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Endonucleases
Escherichia coli
DNA
Divalent Cations
Transfer RNA
DNA Replication
Proteins
Molecules
Salts

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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abstract = "The DNA · protein complex (form I*) of minicircular DNA of Escherichia coli has associated with it an endonucleolytic activity which converts the supercoiled form I into an open circular form II by introducing one single‐stranded break per molecule. This nick can occur with similar frequency in either strand. Prolonged incubation results in the formation of linear duplex molecules (form III). The endonuclease requires a divalent cation (Mg2+ or Mn2+) and is inhibited by high salt concentration and transfer RNA. It might provide a swivel mechanism during the replication of minicircular DNA.",
author = "Joachim Messing",
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AB - The DNA · protein complex (form I*) of minicircular DNA of Escherichia coli has associated with it an endonucleolytic activity which converts the supercoiled form I into an open circular form II by introducing one single‐stranded break per molecule. This nick can occur with similar frequency in either strand. Prolonged incubation results in the formation of linear duplex molecules (form III). The endonuclease requires a divalent cation (Mg2+ or Mn2+) and is inhibited by high salt concentration and transfer RNA. It might provide a swivel mechanism during the replication of minicircular DNA.

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