TY - JOUR
T1 - Engineering E. coli for caffeic acid biosynthesis from renewable sugars
AU - Zhang, Haoran
AU - Stephanopoulos, Gregory
N1 - Funding Information: Acknowledgments This study was supported by ARPA-E (DEAR0000059) provided by US Department of Energy and the Singapore MIT Alliance.
PY - 2013/4
Y1 - 2013/4
N2 - Caffeic acid is a valuable aromatic compound that possesses many important pharmacological activities. In structure, caffeic acid belongs to the hydroxycinnamic acid family and can be biosynthesized from the aromatic amino acid tyrosine. In the present paper, the caffeic acid biosynthesis pathway was reconstituted in engineered Escherichia coli to produce caffeic acid from simple biomass sugar glucose and xylose. Different engineering approaches were utilized to optimize the production. Specifically, two parallel biosynthesis routes leading from tyrosine to caffeic acid were studied. The copy number of the intermediate biosynthesis genes was varied to find appropriate gene doses for caffeic acid biosynthesis. Three different media, including a MOPS medium, a synthetic medium, and a rich medium, were also examined to improve the production. The highest specific caffeic acid production achieved was 38 mg/L/OD. Lastly, cultivation of engineered E. coli in a bioreactor resulted in a production of 106 mg/L caffeic acid after 4 days.
AB - Caffeic acid is a valuable aromatic compound that possesses many important pharmacological activities. In structure, caffeic acid belongs to the hydroxycinnamic acid family and can be biosynthesized from the aromatic amino acid tyrosine. In the present paper, the caffeic acid biosynthesis pathway was reconstituted in engineered Escherichia coli to produce caffeic acid from simple biomass sugar glucose and xylose. Different engineering approaches were utilized to optimize the production. Specifically, two parallel biosynthesis routes leading from tyrosine to caffeic acid were studied. The copy number of the intermediate biosynthesis genes was varied to find appropriate gene doses for caffeic acid biosynthesis. Three different media, including a MOPS medium, a synthetic medium, and a rich medium, were also examined to improve the production. The highest specific caffeic acid production achieved was 38 mg/L/OD. Lastly, cultivation of engineered E. coli in a bioreactor resulted in a production of 106 mg/L caffeic acid after 4 days.
KW - Caffeic acid
KW - Escherichia coli
KW - Heterologous biosynthesis
KW - Tyrosine
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U2 - 10.1007/s00253-012-4544-8
DO - 10.1007/s00253-012-4544-8
M3 - Article
C2 - 23179615
SN - 0175-7598
VL - 97
SP - 3333
EP - 3341
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 8
ER -