Examination of Fe and Cu Isotope Variation in Great Apes Using an Optimized Protocol

TY - JOUR

T1 - Examination of Fe and Cu Isotope Variation in Great Apes Using an Optimized Protocol

AU - Boucher, Renee D

AU - Godfrey, Linda V

AU - Fehren-Schmitz, Lars

AU - Koch, Paul L

N1 - Publisher Copyright: © 2025 John Wiley & Sons Ltd.

PY - 2025/7/30

Y1 - 2025/7/30

N2 - Rationale: Iron deficiency plagues reproductive-aged women across the world, and blood loss during menstruation is proposed as the driving force. To assess if other factors related to reproduction influence Fe and Cu isotope variation in females, we measured Fe and Cu isotope compositions in the bones of chimpanzees and bonobos. Methods: To do this, we optimize the protocol for isolating Fe and Cu (and Zn) from phosphate-rich skeletal materials for further analysis via MC-ICP-MS. Then, we address possible Fe and Cu variation sources in non-menstruating apes (n = 26, of which the sex of 10 was obtained by DNA analysis). Results: The optimized method reduced acid volume by ~14%, and sample preparation time by ~37.5%. We did not find significant sex differences in δ56Fe values (Δ56Fef-m = 0.13‰) or δ65Cu values (Δ65Cuf-m = 0.33‰). Conclusion: Given the similar Δ56Fef-m values between non-menstruating apes and humans, reproductive investment, instead of menstruation alone, is a key factor that drives Fe deficiency in reproductive-aged women and is important to consider with proxies of iron status. Our optimized protocol provides an effective method for exploring iron status in other mammalian species.

AB - Rationale: Iron deficiency plagues reproductive-aged women across the world, and blood loss during menstruation is proposed as the driving force. To assess if other factors related to reproduction influence Fe and Cu isotope variation in females, we measured Fe and Cu isotope compositions in the bones of chimpanzees and bonobos. Methods: To do this, we optimize the protocol for isolating Fe and Cu (and Zn) from phosphate-rich skeletal materials for further analysis via MC-ICP-MS. Then, we address possible Fe and Cu variation sources in non-menstruating apes (n = 26, of which the sex of 10 was obtained by DNA analysis). Results: The optimized method reduced acid volume by ~14%, and sample preparation time by ~37.5%. We did not find significant sex differences in δ56Fe values (Δ56Fef-m = 0.13‰) or δ65Cu values (Δ65Cuf-m = 0.33‰). Conclusion: Given the similar Δ56Fef-m values between non-menstruating apes and humans, reproductive investment, instead of menstruation alone, is a key factor that drives Fe deficiency in reproductive-aged women and is important to consider with proxies of iron status. Our optimized protocol provides an effective method for exploring iron status in other mammalian species.

KW - chimpanzees

KW - estrous

KW - female biology

KW - iron status

KW - reproductive investment

UR - https://www.scopus.com/pages/publications/105003725953

UR - https://www.scopus.com/pages/publications/105003725953#tab=citedBy

U2 - 10.1002/rcm.10051

DO - 10.1002/rcm.10051

M3 - Article

C2 - 40272380

SN - 0951-4198

VL - 39

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 14

M1 - e10051

ER -