Excitation energy transfer in Rhodopseudomonas sphaeroides chromatophore membranes fused with liposomes

Jeffrey D. Pennoyer, Herman J.M. Kramer, Rienk van Grondelle, Willem H.J. Westerhuis, Jan Amesz, Robert Niederman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The role of phospholipid in the structural organization of the light-harvesting complexes of Rhodopseudomonas sphaeroides was examined in photosynthetic (chromatophore) membrane vesicles fused with liposomes. Photochemically active preparations with progressive phospholipid enrichment up to > 15-fold were obtained by both polyethylene glycol- and acidic-pH-induced fusion. Their fluorescence emission at ~ 300 and 77 K was increased by 2-3.5-fold from the peripheral B800-850 antenna relative to that from the core B875 antenna. Up to 30-40% reduction in the efficiency of excitation energy transfer between B850 and B875 was also observed at 77 K suggesting a selective, phospholipid-induced dissociation of a portion of the B800-850 from the rest of the light-harvesting system. Bacterial photosynthesis Bacteriochlorophyll-protein complex Fluorescence spectroscopy Light-harvesting complex Liposome fusion Photosynthetic membrane (Rhodopseudomonas sphaeroides).

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalFEBS Letters
Volume182
Issue number1
DOIs
StatePublished - Mar 11 1985

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Chromatophores
Rhodobacter sphaeroides
Excitation energy
Photosynthetic membranes
Energy Transfer
Liposomes
Energy transfer
Phospholipids
Membranes
Light
Fusion reactions
Antennas
Bacteriochlorophylls
Membrane Fusion
Photosynthesis
Fluorescence Spectrometry
Fluorescence spectroscopy
Fluorescence
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology

Cite this

Pennoyer, Jeffrey D. ; Kramer, Herman J.M. ; van Grondelle, Rienk ; Westerhuis, Willem H.J. ; Amesz, Jan ; Niederman, Robert. / Excitation energy transfer in Rhodopseudomonas sphaeroides chromatophore membranes fused with liposomes. In: FEBS Letters. 1985 ; Vol. 182, No. 1. pp. 145-150.
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abstract = "The role of phospholipid in the structural organization of the light-harvesting complexes of Rhodopseudomonas sphaeroides was examined in photosynthetic (chromatophore) membrane vesicles fused with liposomes. Photochemically active preparations with progressive phospholipid enrichment up to > 15-fold were obtained by both polyethylene glycol- and acidic-pH-induced fusion. Their fluorescence emission at ~ 300 and 77 K was increased by 2-3.5-fold from the peripheral B800-850 antenna relative to that from the core B875 antenna. Up to 30-40{\%} reduction in the efficiency of excitation energy transfer between B850 and B875 was also observed at 77 K suggesting a selective, phospholipid-induced dissociation of a portion of the B800-850 from the rest of the light-harvesting system. Bacterial photosynthesis Bacteriochlorophyll-protein complex Fluorescence spectroscopy Light-harvesting complex Liposome fusion Photosynthetic membrane (Rhodopseudomonas sphaeroides).",
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Excitation energy transfer in Rhodopseudomonas sphaeroides chromatophore membranes fused with liposomes. / Pennoyer, Jeffrey D.; Kramer, Herman J.M.; van Grondelle, Rienk; Westerhuis, Willem H.J.; Amesz, Jan; Niederman, Robert.

In: FEBS Letters, Vol. 182, No. 1, 11.03.1985, p. 145-150.

Research output: Contribution to journalArticle

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AU - Kramer, Herman J.M.

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AU - Amesz, Jan

AU - Niederman, Robert

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AB - The role of phospholipid in the structural organization of the light-harvesting complexes of Rhodopseudomonas sphaeroides was examined in photosynthetic (chromatophore) membrane vesicles fused with liposomes. Photochemically active preparations with progressive phospholipid enrichment up to > 15-fold were obtained by both polyethylene glycol- and acidic-pH-induced fusion. Their fluorescence emission at ~ 300 and 77 K was increased by 2-3.5-fold from the peripheral B800-850 antenna relative to that from the core B875 antenna. Up to 30-40% reduction in the efficiency of excitation energy transfer between B850 and B875 was also observed at 77 K suggesting a selective, phospholipid-induced dissociation of a portion of the B800-850 from the rest of the light-harvesting system. Bacterial photosynthesis Bacteriochlorophyll-protein complex Fluorescence spectroscopy Light-harvesting complex Liposome fusion Photosynthetic membrane (Rhodopseudomonas sphaeroides).

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