Expression of collapsin response mediator proteins in the nervous system of embryonic zebrafish

Jörn Schweitzer, Catherina G. Becker, Melitta Schachner, Thomas Becker

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Collapsin response mediator proteins (CRMPs also known as TUC, Drp, Ulip, TOAD-64) are cytosolic phosphoproteins that are involved in signal transduction during axon growth and in cytoskeletal dynamics. Here we report cloning and mRNA expression patterns of CRMP-1, -2, -3, -4 and, owing to a genome duplication in teleosts, two homologs of CRMP-5 (CRMP-5a and -5b) in embryonic zebrafish at 16 and 24 h post-fertilization (hpf). CRMPs are evolutionarily conserved and zebrafish CRMPs show amino acid identities of 76-90% with their homologs in humans, with the exception of CRMP-3, which shows only 67% homology. Between 16 and 24 hpf, expression of CRMPs generally increased in many regions of the CNS undergoing neuronal differentiation and axonogenesis, but not in the proliferative ventricular zone. Structures that were typically labeled by most, but not all the CRMP probes were the telencephalon, the nucleus of the tract of the post-optic commissure, the epiphysis, the nucleus of the medial longitudinal fascicle, clusters of hindbrain neurons, cranial ganglia, as well as Rohon-Beard neurons. No expression of CRMP mRNAs was observed outside the nervous system. Thus, expression patterns of different CRMP family members correlate with neuronal differentiation and axonogenesis in embryonic zebrafish.

Original languageEnglish (US)
Pages (from-to)809-816
Number of pages8
JournalGene Expression Patterns
Volume5
Issue number6
DOIs
StatePublished - Aug 2005

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Developmental Biology

Keywords

  • Danio rerio
  • Drp
  • Semaphorin
  • TOAD-64
  • TUC
  • Ulip

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