Expression of human cathepsin B protein in Escherichia coli

Marion Man Ying Chan, Dunne Fong

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A cDNA fragment containing the coding sequence for the mature enzyme of human lysosomal proteinase cathepsin B was inserted in the pET plasmid expression vectors, so that it was placed under the control of transcription and translation signals from bacteriophage T7. Upon induction, cathepsin B antigen was detected by in situ immunoscreening of lysed E. coli and by Western blot analysis of bacterial lysates. To our knowledge this is the first report of abundant synthesis of cloned cathepsin B in any expression system. Subfragments of cathepsin B can also be generated by this technique and will be used to study cathepsin B structure and function.

Original languageEnglish (US)
Pages (from-to)219-222
Number of pages4
JournalFEBS Letters
Volume239
Issue number2
DOIs
StatePublished - Nov 7 1988

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology

Keywords

  • (Human)
  • Bacteriophage T
  • Expression vector
  • Gene expression system
  • recombinant DNA

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