Expression of long-term liver-specific function by adult rat hepatocytes cultured on microcarriers

Hein B.A.C. Stockmann, Ronald G. Tompkins, Francois Berthiaume

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The successful development of a bioartificial liver relies, in part, on the ability to maintain viability and differentiated function of a large number of hepatocytes in vitro for extended periods of time. Microcarriers have been widely used to scale up anchorage-dependent mammalian cell cultures. The goal of the present study was to seek optimal culture conditions for hepatocytes attached to microcarriers. Hepatocytes were seeded onto gelatin-coated polystyrene microcarriers, and the composite was cultured in suspension or embedded in an agarose or type I collagen gel for up to 14 days. We were able to seed up to 130 hepatocytes per microcarrier. Viability and function of hepatocytes on microcarriers cultured in suspension or in agarose decreased as a function of time. On the other hand, microcarriers embedded in a collagen gel exhibited specific albumin and urea secretion rates of approximately 3 μg/h/106 cells and 10 μg/h/106 cells during the second week of culture, respectively. These rates were similar to those obtained in control cultures of collagen-sandwiched hepatocytes in the absence of microcarriers. Thus, long-term liver-specific function can be induced by surrounding hepatocytes seeded on microcarriers with a collagen gel. This may be a viable approach to scale up the sandwich hepatocyte culture system.

Original languageEnglish (US)
Pages (from-to)267-279
Number of pages13
JournalTissue Engineering
Volume3
Issue number3
DOIs
StatePublished - Oct 14 1997

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Liver
Rats
Collagen
Gels
Hepatocytes
Cell culture
Urea
Seed
Polystyrenes
Composite materials

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biotechnology
  • Cell Biology

Cite this

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Expression of long-term liver-specific function by adult rat hepatocytes cultured on microcarriers. / Stockmann, Hein B.A.C.; Tompkins, Ronald G.; Berthiaume, Francois.

In: Tissue Engineering, Vol. 3, No. 3, 14.10.1997, p. 267-279.

Research output: Contribution to journalArticle

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