Expression of the Proteus mirabilis lipoprotein gene in Escherichia coli. Existence of tandem promoters

G. Ching, Masayori Inouye

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The lpp gene from Proteus mirabilis was cloned onto pBR322 and expressed in Escherichia coli. The P. mirabilis lpp gene is unique in that it has two tandem promoters transcribing two mRNAs that differ in length by approximately 70 nucleotides at their 5'-ends. The two mRNAs thus encode the identical lipoprotein. The P. mirabilis prolipoprotein has a 19-amino acid signal peptide and a 59-amino acid lipoprotein sequence. In spite of the substantial differences in the amino acid sequence from the E. coli prolipoprotein, the P. mirabilis prolipoprotein is normally modified and processed in E. coli, and the resultant lipoprotein is assembled in the E. coli outer membrane as is the E. coli lipoprotein.

Original languageEnglish (US)
Pages (from-to)4600-4606
Number of pages7
JournalJournal of Biological Chemistry
Volume261
Issue number10
StatePublished - Dec 1 1986
Externally publishedYes

Fingerprint

Proteus mirabilis
Escherichia coli
Lipoproteins
Genes
Amino Acids
Amino Acid Sequence
Messenger RNA
Protein Sorting Signals
Nucleotides
Membranes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

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Expression of the Proteus mirabilis lipoprotein gene in Escherichia coli. Existence of tandem promoters. / Ching, G.; Inouye, Masayori.

In: Journal of Biological Chemistry, Vol. 261, No. 10, 01.12.1986, p. 4600-4606.

Research output: Contribution to journalArticle

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