Expression of type XXIII collagen mRNA and protein

Manuel Koch, Guido Veit, Sigmar Stricker, Pinaki Bhatt, Stefanie Kutsch, Peihong Zhou, Elina Reinders, Rita A. Hahn, Rich Song, Robert E. Burgeson, Donald Gerecke, Stefan Mundlos, Marion Gordon

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Collagen XXIII is a member of the transmembranous subfamily of collagens containing a cytoplasmic domain, a membrane-spanning hydrophobic domain, and three extracellular triple helical collagenous domains interspersed with non-collagenous domains. We cloned mouse, chicken, and human α1(XXIII) collagen cDNAs and showed that this non-abundant collagen has a limited tissue distribution in non-tumor tissues. Lung, cornea, brain, skin, tendon, and kidney are the major sites of expression. In contrast, five transformed cell lines were tested for collagen XXIII expression, and all expressed the mRNA. In vivo the α1(XXIII) mRNA is found in mature and developing organs, the latter demonstrated using stages of embryonic chick cornea and mouse embryos. Polyclonal antibodies were generated in guinea pig and rabbit and showed that collagen XXIII has a transmembranous form and a shed form. Comparison of collagen XXIII with its closest relatives in the transmembranous subfamily of collagens, types XIII and XXV, which have the same number of triple helical and non-collagenous regions, showed that there is a discontinuity in the alignment of domains but that striking similarities remain despite this.

Original languageEnglish (US)
Pages (from-to)21546-21557
Number of pages12
JournalJournal of Biological Chemistry
Volume281
Issue number30
DOIs
StatePublished - Jul 28 2006

Fingerprint

Collagen
Messenger RNA
Proteins
Collagen Type XIII
Cornea
Tissue
Transformed Cell Line
Tendons
Tissue Distribution
Chickens
Brain
Skin
Guinea Pigs
Embryonic Structures
Complementary DNA
Cells
Rabbits
Membranes
Kidney
Lung

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Koch, M., Veit, G., Stricker, S., Bhatt, P., Kutsch, S., Zhou, P., ... Gordon, M. (2006). Expression of type XXIII collagen mRNA and protein. Journal of Biological Chemistry, 281(30), 21546-21557. https://doi.org/10.1074/jbc.M604131200
Koch, Manuel ; Veit, Guido ; Stricker, Sigmar ; Bhatt, Pinaki ; Kutsch, Stefanie ; Zhou, Peihong ; Reinders, Elina ; Hahn, Rita A. ; Song, Rich ; Burgeson, Robert E. ; Gerecke, Donald ; Mundlos, Stefan ; Gordon, Marion. / Expression of type XXIII collagen mRNA and protein. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 30. pp. 21546-21557.
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Koch, M, Veit, G, Stricker, S, Bhatt, P, Kutsch, S, Zhou, P, Reinders, E, Hahn, RA, Song, R, Burgeson, RE, Gerecke, D, Mundlos, S & Gordon, M 2006, 'Expression of type XXIII collagen mRNA and protein', Journal of Biological Chemistry, vol. 281, no. 30, pp. 21546-21557. https://doi.org/10.1074/jbc.M604131200

Expression of type XXIII collagen mRNA and protein. / Koch, Manuel; Veit, Guido; Stricker, Sigmar; Bhatt, Pinaki; Kutsch, Stefanie; Zhou, Peihong; Reinders, Elina; Hahn, Rita A.; Song, Rich; Burgeson, Robert E.; Gerecke, Donald; Mundlos, Stefan; Gordon, Marion.

In: Journal of Biological Chemistry, Vol. 281, No. 30, 28.07.2006, p. 21546-21557.

Research output: Contribution to journalArticle

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T1 - Expression of type XXIII collagen mRNA and protein

AU - Koch, Manuel

AU - Veit, Guido

AU - Stricker, Sigmar

AU - Bhatt, Pinaki

AU - Kutsch, Stefanie

AU - Zhou, Peihong

AU - Reinders, Elina

AU - Hahn, Rita A.

AU - Song, Rich

AU - Burgeson, Robert E.

AU - Gerecke, Donald

AU - Mundlos, Stefan

AU - Gordon, Marion

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Koch M, Veit G, Stricker S, Bhatt P, Kutsch S, Zhou P et al. Expression of type XXIII collagen mRNA and protein. Journal of Biological Chemistry. 2006 Jul 28;281(30):21546-21557. https://doi.org/10.1074/jbc.M604131200