Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM

Jeffrey Haspel, David R. Friedlander, Neely Ivgy-May, Sucheta Chickramane, Chan Roonprapunt, Suzhen Chen, Melitta Camartin, Martin Grumet

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Mammalian LI and avian Ng-CAM arc homologous neural cell adhesion molecules (CAMs) that promote neurite outgrowth and cell adhesion in most neurons. Previous attempts to map these activities to discrete regions in the CAMs have suggested the involvement of a variety of different domains. However, these studies mainly used bacterially expressed proteins that were much less active on a molar basis than the native molecules. To define regions that are critical for maximal neurite outgrowth, we constructed and tested a panel of eukaryotically expressed proteins containing various extracellular segments of human LI (hLl) or Ng-CAM. Our results indicate that Ig domains 1-4 of hLl are critical for homophilic binding and neurite outgrowth; however this segment is less potent than the entire extracellular region. Optimal neurite outgrowth activity was seen with proteins containing all six Ig domains of hLl or Ng-CAM. The adhesive properties of hLl fragments correlated tightly with their neurite outgrowth activities, suggesting that these two processes are closely linked. These results suggest that Ig domains 1-4 form a structural cassette responsible for hLl homophilic binding, while Ig domains 1-6 represent a functional region for optimal promotion of neurite out-growth in vitro and possibly in vivo.

Original languageEnglish (US)
Pages (from-to)287-302
Number of pages16
JournalJournal of Neurobiology
Volume42
Issue number3
StatePublished - Feb 15 2000

Fingerprint

Neural Cell Adhesion Molecule L1
Cell Adhesion Molecules
Neural Cell Adhesion Molecules
Proteins
Neurites
Cell Adhesion
Adhesives
Neuronal Outgrowth
Immunoglobulin Domains
Neurons
Growth

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

Keywords

  • Cell adhesion molecules
  • Ig superfamily
  • Nerve regeneration

Cite this

Haspel, J., Friedlander, D. R., Ivgy-May, N., Chickramane, S., Roonprapunt, C., Chen, S., ... Grumet, M. (2000). Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM. Journal of Neurobiology, 42(3), 287-302.
Haspel, Jeffrey ; Friedlander, David R. ; Ivgy-May, Neely ; Chickramane, Sucheta ; Roonprapunt, Chan ; Chen, Suzhen ; Camartin, Melitta ; Grumet, Martin. / Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM. In: Journal of Neurobiology. 2000 ; Vol. 42, No. 3. pp. 287-302.
@article{7f46859427a3478599671bb339394d5c,
title = "Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM",
abstract = "Mammalian LI and avian Ng-CAM arc homologous neural cell adhesion molecules (CAMs) that promote neurite outgrowth and cell adhesion in most neurons. Previous attempts to map these activities to discrete regions in the CAMs have suggested the involvement of a variety of different domains. However, these studies mainly used bacterially expressed proteins that were much less active on a molar basis than the native molecules. To define regions that are critical for maximal neurite outgrowth, we constructed and tested a panel of eukaryotically expressed proteins containing various extracellular segments of human LI (hLl) or Ng-CAM. Our results indicate that Ig domains 1-4 of hLl are critical for homophilic binding and neurite outgrowth; however this segment is less potent than the entire extracellular region. Optimal neurite outgrowth activity was seen with proteins containing all six Ig domains of hLl or Ng-CAM. The adhesive properties of hLl fragments correlated tightly with their neurite outgrowth activities, suggesting that these two processes are closely linked. These results suggest that Ig domains 1-4 form a structural cassette responsible for hLl homophilic binding, while Ig domains 1-6 represent a functional region for optimal promotion of neurite out-growth in vitro and possibly in vivo.",
keywords = "Cell adhesion molecules, Ig superfamily, Nerve regeneration",
author = "Jeffrey Haspel and Friedlander, {David R.} and Neely Ivgy-May and Sucheta Chickramane and Chan Roonprapunt and Suzhen Chen and Melitta Camartin and Martin Grumet",
year = "2000",
month = "2",
day = "15",
language = "English (US)",
volume = "42",
pages = "287--302",
journal = "Developmental Neurobiology",
issn = "1932-8451",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

Haspel, J, Friedlander, DR, Ivgy-May, N, Chickramane, S, Roonprapunt, C, Chen, S, Camartin, M & Grumet, M 2000, 'Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM', Journal of Neurobiology, vol. 42, no. 3, pp. 287-302.

Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM. / Haspel, Jeffrey; Friedlander, David R.; Ivgy-May, Neely; Chickramane, Sucheta; Roonprapunt, Chan; Chen, Suzhen; Camartin, Melitta; Grumet, Martin.

In: Journal of Neurobiology, Vol. 42, No. 3, 15.02.2000, p. 287-302.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM

AU - Haspel, Jeffrey

AU - Friedlander, David R.

AU - Ivgy-May, Neely

AU - Chickramane, Sucheta

AU - Roonprapunt, Chan

AU - Chen, Suzhen

AU - Camartin, Melitta

AU - Grumet, Martin

PY - 2000/2/15

Y1 - 2000/2/15

N2 - Mammalian LI and avian Ng-CAM arc homologous neural cell adhesion molecules (CAMs) that promote neurite outgrowth and cell adhesion in most neurons. Previous attempts to map these activities to discrete regions in the CAMs have suggested the involvement of a variety of different domains. However, these studies mainly used bacterially expressed proteins that were much less active on a molar basis than the native molecules. To define regions that are critical for maximal neurite outgrowth, we constructed and tested a panel of eukaryotically expressed proteins containing various extracellular segments of human LI (hLl) or Ng-CAM. Our results indicate that Ig domains 1-4 of hLl are critical for homophilic binding and neurite outgrowth; however this segment is less potent than the entire extracellular region. Optimal neurite outgrowth activity was seen with proteins containing all six Ig domains of hLl or Ng-CAM. The adhesive properties of hLl fragments correlated tightly with their neurite outgrowth activities, suggesting that these two processes are closely linked. These results suggest that Ig domains 1-4 form a structural cassette responsible for hLl homophilic binding, while Ig domains 1-6 represent a functional region for optimal promotion of neurite out-growth in vitro and possibly in vivo.

AB - Mammalian LI and avian Ng-CAM arc homologous neural cell adhesion molecules (CAMs) that promote neurite outgrowth and cell adhesion in most neurons. Previous attempts to map these activities to discrete regions in the CAMs have suggested the involvement of a variety of different domains. However, these studies mainly used bacterially expressed proteins that were much less active on a molar basis than the native molecules. To define regions that are critical for maximal neurite outgrowth, we constructed and tested a panel of eukaryotically expressed proteins containing various extracellular segments of human LI (hLl) or Ng-CAM. Our results indicate that Ig domains 1-4 of hLl are critical for homophilic binding and neurite outgrowth; however this segment is less potent than the entire extracellular region. Optimal neurite outgrowth activity was seen with proteins containing all six Ig domains of hLl or Ng-CAM. The adhesive properties of hLl fragments correlated tightly with their neurite outgrowth activities, suggesting that these two processes are closely linked. These results suggest that Ig domains 1-4 form a structural cassette responsible for hLl homophilic binding, while Ig domains 1-6 represent a functional region for optimal promotion of neurite out-growth in vitro and possibly in vivo.

KW - Cell adhesion molecules

KW - Ig superfamily

KW - Nerve regeneration

UR - http://www.scopus.com/inward/record.url?scp=0034651667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034651667&partnerID=8YFLogxK

M3 - Article

C2 - 10645969

VL - 42

SP - 287

EP - 302

JO - Developmental Neurobiology

JF - Developmental Neurobiology

SN - 1932-8451

IS - 3

ER -

Haspel J, Friedlander DR, Ivgy-May N, Chickramane S, Roonprapunt C, Chen S et al. Fast track - Critical and optimal ig domains for promotion of neurite outgrowth by L1/Ng-CAM. Journal of Neurobiology. 2000 Feb 15;42(3):287-302.