Fibrinolytic and coagulant responses to regional limb perfusions of tumor necrosis factor, interferon-γ, and/or melphalan

TY - JOUR

T1 - Fibrinolytic and coagulant responses to regional limb perfusions of tumor necrosis factor, interferon-γ, and/or melphalan

AU - Merryman, Paula

AU - Tannenbaum, Susan H.

AU - Gralnick, Harvey R.

AU - Yu, Kelvin

AU - Arnold, W. Scott

AU - Alexander, H. Richard

AU - Fraker, Douglas

AU - Home, McDonald K.

PY - 1997/1

Y1 - 1997/1

N2 - Regional limb perfusion with antineoplastic agents stresses the local vasculature in a variety of ways. However, by monitoring the perfusates from limbs treated with melphalan alone or with melphalan plus tumor necrosis factor (TNF) and interferon-γ (IFN-γ), we were able to distinguish the effect of the cytokines on the observed coagulant and fibrinolytic responses. We collected samples of effluent from a series of lower extremities that were perfused with the cytokines and/or melphalan as treatment for localized melanoma. Both regimens produced statistically significant evidence of coagulant and fibrinolytic activation. However, limbs receiving cytokines in addition to the melphalan responded with a sharper rise in tissue plasminogen activator (tPA) and plasmin (plasmin-antiplasmin complexes [PAP]) than limbs treated with melphalan alone. Evidence of thrombin formation (prothrombin fragment 1+2 [F1+2], thrombin-antithrombin complexes [TAT]) was also greater when the cytokines were included, although the response was delayed and less consistent than the fibrinolytic activation.

AB - Regional limb perfusion with antineoplastic agents stresses the local vasculature in a variety of ways. However, by monitoring the perfusates from limbs treated with melphalan alone or with melphalan plus tumor necrosis factor (TNF) and interferon-γ (IFN-γ), we were able to distinguish the effect of the cytokines on the observed coagulant and fibrinolytic responses. We collected samples of effluent from a series of lower extremities that were perfused with the cytokines and/or melphalan as treatment for localized melanoma. Both regimens produced statistically significant evidence of coagulant and fibrinolytic activation. However, limbs receiving cytokines in addition to the melphalan responded with a sharper rise in tissue plasminogen activator (tPA) and plasmin (plasmin-antiplasmin complexes [PAP]) than limbs treated with melphalan alone. Evidence of thrombin formation (prothrombin fragment 1+2 [F1+2], thrombin-antithrombin complexes [TAT]) was also greater when the cytokines were included, although the response was delayed and less consistent than the fibrinolytic activation.

UR - https://www.scopus.com/pages/publications/0031059048

UR - https://www.scopus.com/pages/publications/0031059048#tab=citedBy

U2 - 10.1055/s-0038-1655906

DO - 10.1055/s-0038-1655906

M3 - Article

C2 - 9031449

SN - 0340-6245

VL - 77

SP - 53

EP - 56

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

IS - 1

ER -