Fibronectin

a versatile gene for a versatile protein.

R. O. Hynes, Jean E. Schwarzbauer, J. W. Tamkun

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have isolated cDNA and genomic clones for rat fibronectins. A single gene gives rise to three different mRNA species by alternative splicing at a complex intron-exon boundary within the coding region. The fibronectins encoded by these three mRNAs differ by the insertion of different protein segments in the C-terminal heparin-binding domain. The amino acid sequences of the cell-, heparin- and fibrin-binding domains in the C-terminal third of fibronectin were deduced from the DNA sequences. The cell- and heparin-binding regions each consist of several similar repeating sequences known as type III homologies, while the fibrin-binding region comprises three repeats of a different type (type I homologies). The sequences suggest several hypotheses for the structure-function relationships of these domains of fibronectin; these hypotheses are now being tested. Determination of intron-exon boundaries within the fibronectin gene is beginning to reveal the modular structure of the gene and its relation to the repeating structure of fibronectin.

Original languageEnglish (US)
Pages (from-to)75-92
Number of pages18
JournalCiba Foundation symposium
Volume108
StatePublished - Dec 1 1984

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Fibronectins
Genes
Heparin
Proteins
Fibrin
Introns
Exons
Messenger RNA
Alternative Splicing
Amino Acid Sequence
Complementary DNA
Clone Cells

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "We have isolated cDNA and genomic clones for rat fibronectins. A single gene gives rise to three different mRNA species by alternative splicing at a complex intron-exon boundary within the coding region. The fibronectins encoded by these three mRNAs differ by the insertion of different protein segments in the C-terminal heparin-binding domain. The amino acid sequences of the cell-, heparin- and fibrin-binding domains in the C-terminal third of fibronectin were deduced from the DNA sequences. The cell- and heparin-binding regions each consist of several similar repeating sequences known as type III homologies, while the fibrin-binding region comprises three repeats of a different type (type I homologies). The sequences suggest several hypotheses for the structure-function relationships of these domains of fibronectin; these hypotheses are now being tested. Determination of intron-exon boundaries within the fibronectin gene is beginning to reveal the modular structure of the gene and its relation to the repeating structure of fibronectin.",
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Fibronectin : a versatile gene for a versatile protein. / Hynes, R. O.; Schwarzbauer, Jean E.; Tamkun, J. W.

In: Ciba Foundation symposium, Vol. 108, 01.12.1984, p. 75-92.

Research output: Contribution to journalArticle

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