Abstract
Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 967-976 |
| Number of pages | 10 |
| Journal | Journal of Pharmacology and Experimental Therapeutics |
| Volume | 273 |
| Issue number | 2 |
| State | Published - Jan 1 1995 |
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All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Pharmacology
Cite this
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Flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-naphthoflavone. / Stauber, K. L.; Laskin, Jeffrey; Yurkow, Edward; Thomas, P. E.; Laskin, Debra; Conney, A. H.
In: Journal of Pharmacology and Experimental Therapeutics, Vol. 273, No. 2, 01.01.1995, p. 967-976.Research output: Contribution to journal › Article
TY - JOUR
T1 - Flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-naphthoflavone
AU - Stauber, K. L.
AU - Laskin, Jeffrey
AU - Yurkow, Edward
AU - Thomas, P. E.
AU - Laskin, Debra
AU - Conney, A. H.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.
AB - Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.
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M3 - Article
C2 - 7752102
VL - 273
SP - 967
EP - 976
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
SN - 0022-3565
IS - 2
ER -