Flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-naphthoflavone

K. L. Stauber, Jeffrey Laskin, Edward Yurkow, P. E. Thomas, Debra Laskin, A. H. Conney

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Abstract

Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.

Original languageEnglish (US)
Pages (from-to)967-976
Number of pages10
JournalJournal of Pharmacology and Experimental Therapeutics
Volume273
Issue number2
StatePublished - Jan 1 1995

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Cytochromes
Flow Cytometry
Dealkylation
Acetone
Epidermis
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

@article{73494bb7daf54401b105f17a630dbc23,
title = "Flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-naphthoflavone",
abstract = "Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50{\%} to 60{\%} of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70{\%} of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12{\%} were suprabasal differentiated cells. In contrast, about 50{\%} of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35{\%} were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80{\%} of the separated suprabasal cells and in about 35{\%} of the separated basal cells.",
author = "Stauber, {K. L.} and Jeffrey Laskin and Edward Yurkow and Thomas, {P. E.} and Debra Laskin and Conney, {A. H.}",
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T1 - Flow cytometry reveals subpopulations of murine epidermal cells that are refractory to induction of cytochrome P-4501A1 by β-naphthoflavone

AU - Stauber, K. L.

AU - Laskin, Jeffrey

AU - Yurkow, Edward

AU - Thomas, P. E.

AU - Laskin, Debra

AU - Conney, A. H.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.

AB - Topical application of β-naphthoflavone to CD-1 mice induced an 87-fold increase in epidermal 7-ethoxyresorufin O-dealkylation activity per cell and a many-fold increase in epidermal cytochrome P-4501A1 (CYP1A1) concentration. Flow cytometric analysis of individual epidermal cells from acetone-treated and β-naphthoflavone-treated mice using a monoclonal antibody for CYP1A1 indicated that 50% to 60% of the isolated epidermal cells were refractory to β-naphthoflavone induction of CYP1A1. Examination of the differences between responsive and nonresponsive epidermal cells from β-naphthoflavone-treated mice revealed that 70% of the low CYP1A1-containing cells (noninduced) separated by flow cytometry were basal cells and only 12% were suprabasal differentiated cells. In contrast, about 50% of the high CYP1A1-containing induced cells separated by flow cytometry from the epidermis of mice treated with β-naphthoflavone were suprabasal cells and 35% were basal cells. These results indicate that topical application of β-naphthoflavone increased the level of CYP1A1 in about 80% of the separated suprabasal cells and in about 35% of the separated basal cells.

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