TY - JOUR
T1 - Fluorescent methods to study transcription initiation and transition into elongation.
AU - Deshpande, Aishwarya P.
AU - Sultana, Shemaila
AU - Patel, Smita S.
PY - 2014
Y1 - 2014
N2 - The DNA-dependent RNA polymerases induce specific conformational changes in the promoter DNA during transcription initiation. Fluorescence spectroscopy sensitively monitors these DNA conformational changes in real time and at equilibrium providing powerful ways to estimate interactions in transcriptional complexes and to assess how transcription is regulated by the promoter DNA sequence, transcription factors, and small ligands. Ensemble fluorescence methods described here probe the individual steps of promoter binding, bending, opening, and transition into the elongation using T7 phage and mitochondrial transcriptional systems as examples.
AB - The DNA-dependent RNA polymerases induce specific conformational changes in the promoter DNA during transcription initiation. Fluorescence spectroscopy sensitively monitors these DNA conformational changes in real time and at equilibrium providing powerful ways to estimate interactions in transcriptional complexes and to assess how transcription is regulated by the promoter DNA sequence, transcription factors, and small ligands. Ensemble fluorescence methods described here probe the individual steps of promoter binding, bending, opening, and transition into the elongation using T7 phage and mitochondrial transcriptional systems as examples.
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U2 - https://doi.org/10.1007/978-3-0348-0856-9_6
DO - https://doi.org/10.1007/978-3-0348-0856-9_6
M3 - Review article
C2 - 25095993
VL - 105
SP - 105
EP - 130
JO - EXS
JF - EXS
SN - 1023-294X
ER -