Fluorescent molecular rotors as versatile in situ sensors for protein quantitation

Kevin Daus; Sorachat Tharamak; Wanchai Pluempanupat; Peter A. Galie; Maria A. Theodoraki; Emmanuel A. Theodorakis; Mary L. Alpaugh

doi:10.1038/s41598-023-46571-5

Fluorescent molecular rotors as versatile in situ sensors for protein quantitation

Kevin Daus, Sorachat Tharamak, Wanchai Pluempanupat, Peter A. Galie, Maria A. Theodoraki, Emmanuel A. Theodorakis, Mary L. Alpaugh

Rowan University

Research output: Contribution to journal › Article › peer-review

Abstract

Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.

Original language	American English
Article number	20529
Journal	Scientific reports
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41598-023-46571-5
State	Published - Dec 2023

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abstract = "Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.",

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AU - Tharamak, Sorachat

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AU - Theodoraki, Maria A.

AU - Theodorakis, Emmanuel A.

AU - Alpaugh, Mary L.

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AB - Accurate protein quantitation is essential for many cellular mechanistic studies. Existing technology relies on extrinsic sample evaluation that requires significant volumes of sample as well as addition of assay-specific reagents and importantly, is a terminal analysis. This study exploits the unique chemical features of a fluorescent molecular rotor that fluctuates between twisted-to-untwisted states, with a subsequent intensity increase in fluorescence depending on environmental conditions (e.g., viscosity). Here we report the development of a rapid, sensitive in situ protein quantitation method using ARCAM-1, a representative fluorescent molecular rotor that can be employed in both non-terminal and terminal assays.

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Fluorescent molecular rotors as versatile in situ sensors for protein quantitation

Abstract

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