Functional analysis of the subunits of the chromatin assembly factor RSF

Alejandra Loyola, Jing Yi Huang, Gary LeRoy, Sherrie Hu, Yuh Hwa Wang, Robert Donnelly, William S. Lane, Sheng Chung Lee, Danny Reinberg

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Abstract

The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.

Original languageEnglish (US)
Pages (from-to)6759-6768
Number of pages10
JournalMolecular and cellular biology
Volume23
Issue number19
DOIs
StatePublished - Oct 1 2003

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Chromatin Assembly and Disassembly
Nucleosomes
Hepatitis B virus
Genes
Adenosine Triphosphatases
Dissection
Organism Cloning
Proteins
Complementary DNA
Amino Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Loyola, Alejandra ; Huang, Jing Yi ; LeRoy, Gary ; Hu, Sherrie ; Wang, Yuh Hwa ; Donnelly, Robert ; Lane, William S. ; Lee, Sheng Chung ; Reinberg, Danny. / Functional analysis of the subunits of the chromatin assembly factor RSF. In: Molecular and cellular biology. 2003 ; Vol. 23, No. 19. pp. 6759-6768.
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abstract = "The human ISWI-containing factor RSF (for remodeling and spacing factor) is composed of two subunits: the ATPase hSNF2H and p325 (Rsf-1), a protein encoded by a novel human gene. We previously showed that RSF mediates nucleosome deposition and generates regularly spaced nucleosome arrays. Here we report the characterization of the largest subunit of RSF, Rsf-1. We found that Rsf-1 is a highly acidic protein containing a plant homology domain. The present study includes the cloning of Rsf-1, the preparation of recombinant RSF, and the dissection of the role of each subunit in the chromatin assembly reaction. The sequence of the gene for Rsf-1 includes a recently characterized cDNA, HBXAP; postulated to be involved in the transcriptional regulation of the hepatitis B virus. HBXAP actually contains a 252-amino-acid truncation of the amino terminus of Rsf-1. Finally, comparison of HBXAP and Rsf-1 properties shows that they are functionally different.",
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Loyola, A, Huang, JY, LeRoy, G, Hu, S, Wang, YH, Donnelly, R, Lane, WS, Lee, SC & Reinberg, D 2003, 'Functional analysis of the subunits of the chromatin assembly factor RSF', Molecular and cellular biology, vol. 23, no. 19, pp. 6759-6768. https://doi.org/10.1128/MCB.23.19.6759-6768.2003

Functional analysis of the subunits of the chromatin assembly factor RSF. / Loyola, Alejandra; Huang, Jing Yi; LeRoy, Gary; Hu, Sherrie; Wang, Yuh Hwa; Donnelly, Robert; Lane, William S.; Lee, Sheng Chung; Reinberg, Danny.

In: Molecular and cellular biology, Vol. 23, No. 19, 01.10.2003, p. 6759-6768.

Research output: Contribution to journalArticle

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AU - Reinberg, Danny

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