Functional characterization of human alloreactive T cell clones

Christine Rohowsky-Kochan; Vincent Bonagura; Amy Lewison; Donald West King; Nicole Suciu-Foca

doi:10.1016/0198-8859(84)90057-0

Functional characterization of human alloreactive T cell clones

Christine Rohowsky-Kochan
, Vincent Bonagura
, Amy Lewison
, Donald West King
, Nicole Suciu-Foca

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

We have investigated the functional activity of alloreactive T cell clones, generated by limiting dilutions of MLC blasts. Clones displaying helper, suppressor, or cytotoxic functions (against Class I or Class II HLA antigens) were identified, using proliferative, ⁵¹Cr-release, and hemolytique-plaque induction assays. To determine whether functionally distinct clones share determinants that trigger the AMLC reaction we have tested the capacity of selected clones to cross-stimulate each other in secondary AMLC. Our data suggest that AMLC stimulatory determinants are related to common structure antigens of the T cell receptor for alloantigen, since helper clones, for example, (TLC #19) can induce memory responses to suppressor (TLC #209) as well as cytotoxic (TLC #207) clones and vice versa. The AMLC systems seems well suited for studying network regulations within the T cell compartment.

Original language	American English
Pages (from-to)	173-182
Number of pages	10
Journal	Human Immunology
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/0198-8859(84)90057-0
State	Published - Dec 1984
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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@article{297ec188392f4076aaaf257c61255629,

title = "Functional characterization of human alloreactive T cell clones",

abstract = "We have investigated the functional activity of alloreactive T cell clones, generated by limiting dilutions of MLC blasts. Clones displaying helper, suppressor, or cytotoxic functions (against Class I or Class II HLA antigens) were identified, using proliferative, 51Cr-release, and hemolytique-plaque induction assays. To determine whether functionally distinct clones share determinants that trigger the AMLC reaction we have tested the capacity of selected clones to cross-stimulate each other in secondary AMLC. Our data suggest that AMLC stimulatory determinants are related to common structure antigens of the T cell receptor for alloantigen, since helper clones, for example, (TLC \#19) can induce memory responses to suppressor (TLC \#209) as well as cytotoxic (TLC \#207) clones and vice versa. The AMLC systems seems well suited for studying network regulations within the T cell compartment.",

author = "Christine Rohowsky-Kochan and Vincent Bonagura and Amy Lewison and King, \{Donald West\} and Nicole Suciu-Foca",

note = "Funding Information: We thank Violet Aponte for her secretarial assistance. This work was supported by grants NO1-AI-32505 and 1-POL-AGO-2307 from the NIH. OKT antibodies were a generous gift from Ortho Diagnostics Laboratories, Raritan, NJ.",

year = "1984",

month = dec,

doi = "10.1016/0198-8859(84)90057-0",

language = "American English",

volume = "11",

pages = "173--182",

journal = "Human Immunology",

issn = "0198-8859",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Functional characterization of human alloreactive T cell clones

AU - Rohowsky-Kochan, Christine

AU - Bonagura, Vincent

AU - Lewison, Amy

AU - King, Donald West

AU - Suciu-Foca, Nicole

N1 - Funding Information: We thank Violet Aponte for her secretarial assistance. This work was supported by grants NO1-AI-32505 and 1-POL-AGO-2307 from the NIH. OKT antibodies were a generous gift from Ortho Diagnostics Laboratories, Raritan, NJ.

PY - 1984/12

Y1 - 1984/12

N2 - We have investigated the functional activity of alloreactive T cell clones, generated by limiting dilutions of MLC blasts. Clones displaying helper, suppressor, or cytotoxic functions (against Class I or Class II HLA antigens) were identified, using proliferative, 51Cr-release, and hemolytique-plaque induction assays. To determine whether functionally distinct clones share determinants that trigger the AMLC reaction we have tested the capacity of selected clones to cross-stimulate each other in secondary AMLC. Our data suggest that AMLC stimulatory determinants are related to common structure antigens of the T cell receptor for alloantigen, since helper clones, for example, (TLC #19) can induce memory responses to suppressor (TLC #209) as well as cytotoxic (TLC #207) clones and vice versa. The AMLC systems seems well suited for studying network regulations within the T cell compartment.

AB - We have investigated the functional activity of alloreactive T cell clones, generated by limiting dilutions of MLC blasts. Clones displaying helper, suppressor, or cytotoxic functions (against Class I or Class II HLA antigens) were identified, using proliferative, 51Cr-release, and hemolytique-plaque induction assays. To determine whether functionally distinct clones share determinants that trigger the AMLC reaction we have tested the capacity of selected clones to cross-stimulate each other in secondary AMLC. Our data suggest that AMLC stimulatory determinants are related to common structure antigens of the T cell receptor for alloantigen, since helper clones, for example, (TLC #19) can induce memory responses to suppressor (TLC #209) as well as cytotoxic (TLC #207) clones and vice versa. The AMLC systems seems well suited for studying network regulations within the T cell compartment.

UR - https://www.scopus.com/pages/publications/0021705744

UR - https://www.scopus.com/pages/publications/0021705744#tab=citedBy

U2 - 10.1016/0198-8859(84)90057-0

DO - 10.1016/0198-8859(84)90057-0

M3 - Article

C2 - 6239844

SN - 0198-8859

VL - 11

SP - 173

EP - 182

JO - Human Immunology

JF - Human Immunology

IS - 4

ER -

Functional characterization of human alloreactive T cell clones

Abstract

ASJC Scopus subject areas

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