Functional characterization of interstitial macrophages and subpopulations of alveolar macrophages from rat lung

S. Prokhorova, N. Lavrikova, D. L. Laskin

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The specific function of interstitial macrophages (IM) in the lung is poorly understood because of difficulties in isolating these cells in high purity or large number. In the present studies, a pure population of enzymatically isolated IM and lung macrophages obtained mechanically from the lung were compared functionally with alveolar macrophages recovered by lavage (AM). Macrophages isolated mechanically from the tissue and AM displayed similarly high levels of Fc-receptor mediated phagocytosis. In contrast, IM phagocytized significantly fewer opsonized sheep red blood cells per macrophage than AM. In addition, although some variations in the amounts of nitric oxide and superoxide anion produced by AM and macrophages obtained by mechanical tissue disruption were observed, these subpopulations released significantly more of these mediators than IM. These data support the concept that macrophages isolated by mechanical disruption of the tissue represent a subpopulation of AM. We also found that, in contrast to AM, IM did not respond synergistically to combinations of IFN-γ and lipopolysaccharide (LPS) or tumor necrosis factor α in terms of nitric oxide production. Furthermore, regulation of superoxide anion release in AM and IM by LPS and/or IFN-γ was distinct. Taken together, these studies demonstrate that IM are functionally different from other macrophage subpopulations which might reflect their unique location within the lung.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalJournal of Leukocyte Biology
Issue number2
StatePublished - 1994

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


  • IFN-γ
  • lipopolysaccharide
  • nitric oxide
  • phagocytosis
  • superoxide anion


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