Functional coupling of the 5-HT2C serotonin receptor to G proteins in Xenopus oocytes

Yan Chen, Melvyn Baez, Lei Yu

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

The serotonin 2c (5-HT2C) receptor mediates its cellular effects by interacting with heterotrimeric guanine nucleotide binding proteins (G proteins). To characterize which G proteins are involved in functional coupling to the receptor, a mouse 5-HT2C receptor was expressed in Xenopus oocytes, and antisense oligonucleotides complementary to the mRNA sequence of the endogenous Xenopus G protein α subunits were used to inhibit G protein synthesis. Antisense oligonucleotide against the Xenopus Go α subunit inhibited the 5-HT2C receptor function, and coexpression of a rat Go α subunit reversed the inhibition by the anti-Xenopus Go oligonucleotide. Furthermore, antisense oligonucleotides against both the Go and Gil α subunits inhibited the electrophysiologic response induced by stimulation of the 5-HT2C receptor. These data suggest that both Go and Gil are involved in functional coupling of the 5-HT2C receptor to phospholipase C in Xenopus oocytes.

Original languageEnglish (US)
Pages (from-to)100-102
Number of pages3
JournalNeuroscience Letters
Volume179
Issue number1-2
DOIs
StatePublished - Sep 26 1994
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

Keywords

  • Antisense inhibition
  • G protein
  • Serotonin receptor
  • Xenopus oocyte

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