Generation and Characterization of Anti-Idiotypic Antibodies Recognizing the Interferon-α Receptor: Implications for Ligand–Receptor Interactions

Jin Kyu Lim, Jerome A. Langer

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Monoclonal antibodies LI-1 and LI-8 against interferon-αA (IFN-α A) block IFN-αA activity and binding to its receptor, but they recognize distinct epitopes. Surprisingly, anti-idiotypic antibodies to both LI-1 and LI-8 have properties consistent with recognition of the receptor: anti-LI-1 and anti-LI-8 antibodies inhibit the binding of IFN-αA to its receptor. However, anti-LI-1 is an antagonist of IFN-αA, while anti-LI-8 is an agonist. Thus, at least some part of the epitopes on IFN-αA recognized by LI-1 and LI-8 are directly involved in receptor binding. Because these epitopes are spatially distinct, the implication is that the receptor binding site on IFN-αA must be extensive, or there are minimally two regions of IFN-αA involved in receptor interactions.

Original languageEnglish (US)
Pages (from-to)295-301
Number of pages7
JournalJournal of Interferon Research
Volume13
Issue number4
DOIs
StatePublished - Aug 1993

All Science Journal Classification (ASJC) codes

  • Virology
  • Immunology

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